Goat anti-Guinea Pig IgG Fc Secondary Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60685
Conjugate
Catalog #
Key Product Details
Species Reactivity
Guinea Pig
Applications
Dot Blot, ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Guinea Pig IgG F(c) fragment
Specificity
No reaction was observed against Guinea Pig IgG F(ab).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Guinea Pig IgG, Guinea Pig IgG F(c) and Guinea Pig Serum
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Goat anti-Guinea Pig IgG Fc Secondary Antibody
Dot Blot: Goat anti-Guinea Pig IgG Fc Secondary Antibody [NBP2-60685] - Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Goat anti-Guinea Pig IgG AntibodyAlkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Blocked with blocking buffer for 60 min at RT. Visualized using Alkaline Phosphatase Substrate for 30 seconds at RT. Image from the Alkaline Phosphatase version of this antibody.
Applications for Goat anti-Guinea Pig IgG Fc Secondary Antibody
Application
Recommended Usage
ELISA
1:20000 - 1:100000
Immunohistochemistry
1:1000-1:5000
Western Blot
1:2000-1:10000
Application Notes
This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for Anti-guinea Pig IgG F(c) Antibody.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Goat anti-Guinea Pig IgG Fc Secondary Antibody
Product Specific Notices for Goat anti-Guinea Pig IgG Fc Secondary Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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