Chicken anti-Rabbit IgG (H+L) Secondary Antibody [PE/Cy7]

Novus Biologicals, part of Bio-Techne | Catalog # NB120-6831PECY7

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Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Unconjugated

NB120-6831

Alkaline Phosphatase

NB120-6830

Allophycocyanin

NB120-6831APC

Allophycocyanin/Cy7

NB120-6831APCCY7

Biotin

NBP1-73443

DyLight 350

NB120-6831UV

DyLight 405

NB120-6831V

DyLight 488

NB120-6831G

DyLight 550

NB120-6831R

DyLight 594

NB120-6831DL594

DyLight 650

NB120-6831C

DyLight 680

NB120-6831FR

DyLight 755

NB120-6831IR

FITC

NBP1-73333

HRP

NBP1-73543

Janelia Fluor 549

NB120-6831JF549

Janelia Fluor 646

NB120-6831JF646

PE

NB120-6831PE

PE/Atto594

NB120-6831PEATT594

PE/Cy5.5

NB120-6831PECY55

PerCP

NB120-6831PCP

Rhodamine

NB120-6826

Texas Red

NBP1-73444

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Rabbit

Applications

Chromatin Immunoprecipitation (ChIP), Dot Blot, ELISA, Immunohistochemistry, Western Blot

Label

PE/Cy7 (Excitation = 488 nm, Emission = 778 nm)

Antibody Source

Polyclonal Chicken IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgG (H+L) Secondary Antibodies »

Product Summary for Chicken anti-Rabbit IgG (H+L) Secondary Antibody [PE/Cy7]

Immunogen

Rabbit IgG whole molecule

Clonality

Polyclonal

Host

Chicken

Isotype

IgG

Description

Chicken products cannot be exported to Canada.

Applications for Chicken anti-Rabbit IgG (H+L) Secondary Antibody [PE/Cy7]

Application

Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

Dot Blot

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: IgG (H+L)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Additional IgG (H+L) Products

Product Documents for Chicken anti-Rabbit IgG (H+L) Secondary Antibody [PE/Cy7]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Chicken anti-Rabbit IgG (H+L) Secondary Antibody [PE/Cy7]

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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