Rabbit anti-Rat IgG (H+L) Secondary Antibody [DyLight 650]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-75412C
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
ELISA, Immunoassay
Label
DyLight 650 (Excitation = 652 nm, Emission = 672 nm)
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This Rabbit anti-Rat IgG (H+L) Secondary Antibody was developed against purified rat IgG (H&L).
Specificity
Based on IEP, this Rabbit anti-Rat IgG (H+L) Secondary Antibody reacts with heavy gamma chains on rat IgG and light chains on all rat immunoglobulins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Applications for Rabbit anti-Rat IgG (H+L) Secondary Antibody [DyLight 650]
Application
Recommended Usage
ELISA
1:100-1:2000
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: IgG (H+L)
The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).
References
1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211
2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78
3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268
4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619
Additional IgG (H+L) Products
Product Documents for Rabbit anti-Rat IgG (H+L) Secondary Antibody [DyLight 650]
Product Specific Notices for Rabbit anti-Rat IgG (H+L) Secondary Antibody [DyLight 650]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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