Donkey anti-Sheep IgG (H+L) Secondary Antibody [DyLight 650] (Pre-adsorbed)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-75675
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Sheep
Applications
Flow Cytometry, Immunomicroscopy
Label
DyLight 650 (Excitation = 652 nm, Emission = 672 nm)
Antibody Source
Polyclonal Donkey IgG
Format
Pre-adsorbed
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This Donkey anti-Sheep IgG (H+L) Secondary Antibody [DyLight 650] (Pre-adsorbed) was developed against purified sheep IgG (H&L).
Reactivity Notes
Based on IEP, no reactivity is observed to non-immunoglobulin sheep serum proteins, IgG from human or rabbit, and serum proteins from human or rabbit
Specificity
Based on IEP, this Donkey anti-Sheep IgG (H+L) Secondary Antibody [DyLight 650] (Pre-adsorbed) heavy gamma chains on sheep IgG and light chains on all sheep immunoglobulins. This antibody has been pre-adsorbed against human or rabbit IgG
Clonality
Polyclonal
Host
Donkey
Isotype
IgG
Description
Affinity purified antibody is > 95% based on SDS-PAGE. Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. DyLight 650 (Ex = 652 nm; Em = 672 nm). After reconstituted, prepare fresh working dilution daily
Product is stable for up to 4 weeks at 2-8C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C.
Product is stable for up to 4 weeks at 2-8C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C.
Applications
Application
Recommended Usage
Flow Cytometry
1:20 - 1:2000
Immunomicroscopy
1:20 - 1:2000
Application Notes
This conjugate is suitable for immunomicroscopy and flow cytometry.
Formulation, Preparation, and Storage
Purification
Affinity purified
Reconstitution
Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Formulation
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Format
Pre-adsorbed
Preservative
0.05% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.
Background: IgG (H+L)
The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).
References
1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211
2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78
3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268
4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619
Additional IgG (H+L) Products
Product Specific Notices
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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