Rabbit anti-Human IgG Heavy Chain Secondary Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22138
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Dot Blot, ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Human IgG gamma heavy chain
Specificity
No reaction was observed against Human IgM or Human IgA. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG and Human Serum
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Rabbit anti-Human IgG Heavy Chain Secondary Antibody
Dot Blot: Rabbit anti-Human IgG Heavy Chain Secondary Antibody [NBP2-22138] - Dot Blot of Rabbit anti-Human IgG Secondary Antibody [Alkaline Phosphatase]. Antigen: Human IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Incubated for 1hr at RT; blocked for 60 min at RT. Image using the Alkaline Phosphatase form of this antibody.
Applications for Rabbit anti-Human IgG Heavy Chain Secondary Antibody
Application
Recommended Usage
ELISA
1:20000 - 1:100000
Immunohistochemistry
1:1000-1:5000
Western Blot
1:2000-1:10000
Application Notes
This product is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG Heavy Chain
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Heavy Chain Products
Product Documents for Rabbit anti-Human IgG Heavy Chain Secondary Antibody
Product Specific Notices for Rabbit anti-Human IgG Heavy Chain Secondary Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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