Rabbit anti-Rat IgG2b Secondary Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69611
Key Product Details
Species Reactivity
Rat
Applications
ELISA, Immunoelectrophoresis, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Pools of purified homogenous IgG2b isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Reactivity Notes
This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this product has not been tested in detail, However in double radial immunodiffusion a reaction with Mouse has been observed.
Specificity
The reactivity of the antiserum is directed to the Fc portion of the subclass IgG2b. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Clonality
Polyclonal
Host
Rabbit
Description
The lyophilized antiserum is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4C, not refrozen, and preferably used the same day.
Applications for Rabbit anti-Rat IgG2b Secondary Antibody
Application
Recommended Usage
Immunoprecipitation
1:10 - 1:500
Application Notes
Precipitation assays. In immunoelectrophoresis use 2 ul or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in a 3 mm diameter center well and 2 ul serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Precipitation assays.
Precipitation assays.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Whole Antiserum
Reconstitution
Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2).
Formulation
Whole antiserum
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG2B
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G2B Fc
Additional IgG2B Products
Product Documents for Rabbit anti-Rat IgG2b Secondary Antibody
Product Specific Notices for Rabbit anti-Rat IgG2b Secondary Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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