Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgG2b Heavy Chain Secondary Antibodies »

Product Specifications

Immunogen

Mouse IgG2b heavy chain

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum and Mouse IgG.

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody

Western Blot: Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody [NBP1-72795]

Western Blot: Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody [NBP1-72795]

Western Blot: Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody [NBP1-72795] - Mouse IgG2b. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.
Immunocytochemistry/ Immunofluorescence: Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody [NBP1-72795]

Immunocytochemistry/ Immunofluorescence: Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody [NBP1-72795]

Immunocytochemistry/Immunofluorescence: Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody [NBP1-72795] - Immunocytochemistry/Immunofluorescence: Rabbit anti-Mouse IgG2b Secondary Antibody [DyLight 488] [NBP1-72892] Image from the DyLight 488 version of this antibody.
Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody [NBP1-72795]

Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody [NBP1-72795]

Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody [NBP1-72795] - Rabbit anti-Mouse IgG2b Antibody [DyLight 800] [NBP1-72916]
View 1 more images

Applications for Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody

Application
Recommended Usage

ELISA

1:20000 - 1:100000

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Frozen

1:1000 - 1:5000

Western Blot

1:2000 - 1:10000
Application Notes
This product has been tested by ELISA and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents for Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rabbit anti-Mouse IgG2b Heavy Chain Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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