Rabbit anti-Mouse IgM Fc Secondary Antibody

Mouse

Dot Blot, ELISA, Western Blot

Unconjugated

Polyclonal Rabbit

LYOPH mg/ml

Highly purified homogenous IgM isolated from mouse serum. Immunization with intact (19S) and split IgM (7S). Freund's complete adjuvant is used in the first step of the immunization procedure.

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since immunoglobulins of different species frequently share antigenic determinants. Cross-reactivity of this product has not been tested in detail.

The defined antibody specificity is restricted to the Fc part of the IgM molecule, as tested against mouse sera at the level of sensitivity of direct immunofluorescence staining methods applied to lymphoid cells with a FITC conjugated form of this product. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations, against mouse serum a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with the purified IgM. It does not react with IgG, IgG/Fab fragments and IgA.

Polyclonal

Rabbit

The lyophilized product is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Ion exchange chromatography

Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.

Lyophilized from PBS (pH 7.2)

No Preservative

LYOPH mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.