Goat anti-Mouse IgM Heavy Chain Secondary Antibody [DyLight 800]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72884
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
DyLight 800 (Excitation = 777 nm, Emission = 794 nm)
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Mouse IgM whole molecule
Specificity
No reaction was observed against other mouse heavy or light chain proteins.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum
Scientific Data Images for Goat anti-Mouse IgM Heavy Chain Secondary Antibody [DyLight 800]
Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [DyLight 800] [NBP1-72884] - Fluorescence absorption and emission spectra of Dylight 800 in PBS, pH 7.2.
Goat anti-Mouse IgM Heavy Chain Secondary Antibody [DyLight 800]
DyLight(TM) dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight(TM) 680 conjugate. Anti-TNFa was detected using a DyLight(TM) 800 conjugate. The image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR.Applications for Goat anti-Mouse IgM Heavy Chain Secondary Antibody [DyLight 800]
Application
Recommended Usage
Fluorophore-linked immunosorbent assay
1:20000
Immunocytochemistry/ Immunofluorescence
1:5000
Western Blot
1:10000
Application Notes
The emission spectra for this DyLight(TM) conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgM Heavy Chain
Alternate Names
Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain
Gene Symbol
IGHM
Additional IgM Heavy Chain Products
Product Documents for Goat anti-Mouse IgM Heavy Chain Secondary Antibody [DyLight 800]
Product Specific Notices for Goat anti-Mouse IgM Heavy Chain Secondary Antibody [DyLight 800]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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