Rabbit anti-Rat IgM Heavy Chain Secondary Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73724
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Label
HRP
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Rat IgM whole molecule
Specificity
No reaction was observed against other rat heavy or light chain proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgM and Rat Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgM and Rat Serum
Scientific Data Images for Rabbit anti-Rat IgM Heavy Chain Secondary Antibody [HRP]
ELISA: Rabbit anti-Rat IgM Heavy Chain Secondary Antibody [HRP] [NBP1-73724] - ELISA Results of Rabbit anti-Rat IgM Heavy Chain Heavy Chain Secondary antibody [HRP] tested against purified Rat IgM Heavy Chain HRP. Each well was coated in duplicate with 1.0 ug of Rat IgM Heavy Chain. The working dilution is 1:62,800. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer and TMB substrate.
Applications for Rabbit anti-Rat IgM Heavy Chain Secondary Antibody [HRP]
Application
Recommended Usage
ELISA
1:30000 - 1:70000
Immunohistochemistry
1:500 - 1:2500
Western Blot
1:1000 - 1:5000
Application Notes
This product has been tested by ELISA and is suitable for ELISA, Western Blot and Immunohistochemistry. Antibody should be optimized by the end user for specific reactive conditions.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgM Heavy Chain
Alternate Names
Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain
Gene Symbol
IGHM
Additional IgM Heavy Chain Products
Product Documents for Rabbit anti-Rat IgM Heavy Chain Secondary Antibody [HRP]
Product Specific Notices for Rabbit anti-Rat IgM Heavy Chain Secondary Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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