Key Product Details

Species Reactivity

Chicken

Applications

ELISA, Immunohistochemistry, SDS-Page, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgM Heavy Chain Secondary Antibodies »

Product Specifications

Immunogen

Chicken IgM whole molecule

Specificity

No reaction was observed against other Chicken heavy or light chain proteins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgM and Chicken Serum

Store at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Goat anti-Chicken IgM Heavy Chain Secondary Antibody

Western Blot: Goat anti-Chicken IgM Heavy Chain Secondary Antibody [NBP2-60690]

Western Blot: Goat anti-Chicken IgM Heavy Chain Secondary Antibody [NBP2-60690]

Western Blot: Goat anti-Chicken IgM Heavy Chain Secondary Antibody [NBP2-60690] - Lane 1: Chicken IgG whole molecule. Lane 2: Chicken IgG F(c) Fragment. Lane 3: Chicken IgG F(ab) Fragment. Lane 4: Chicken IgM Whole Molecule. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Chicken IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated 1:30,000 for 60 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgG, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher. Image using the Biotin form of this antibody.
SDS-PAGE: Goat anti-Chicken IgM Heavy Chain Secondary Antibody [NBP2-60690]

SDS-PAGE: Goat anti-Chicken IgM Heavy Chain Secondary Antibody [NBP2-60690]

SDS-Page: Goat anti-Chicken IgM Heavy Chain Secondary Antibody [NBP2-60690] - Lane 1: Goat anti-Chicken IgM Secondary Antibody - Non-Reduced. Lane 2: Goat anti-Chicken IgM Secondary Antibody - Reduced. Load: 1.0 ug per lane. Predicted/Observed size: 50 kDa, 25 kDa for Reduced - Goat IgG. Other band(s): none.

Applications for Goat anti-Chicken IgM Heavy Chain Secondary Antibody

Application
Recommended Usage

ELISA

1:20000 - 1:100000

Immunohistochemistry

1:1000-1:5000

Western Blot

1:2000-1:10000
Application Notes
This product has been tested by ELISA and SDS-PAGE and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgM Heavy Chain

Alternate Names

Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain

Gene Symbol

IGHM

Additional IgM Heavy Chain Products

Product Documents for Goat anti-Chicken IgM Heavy Chain Secondary Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Goat anti-Chicken IgM Heavy Chain Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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