Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Lambda Light Chain Secondary Antibodies »

Product Specifications

Immunogen

Goat anti-Human Lambda Light Chain Secondary Antibody was produced by repeated immunization with Human lambda light chain molecule in goat.

Specificity

Specificity was confirmed by ELISA.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. Solid phase adsorption(s) against a panel of Human IgG Kappa, IgM Kappa and IgA Kappa proteins was performed

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Goat anti-Human Lambda Light Chain Secondary Antibody

Western Blot: Goat anti-Human Lambda Light Chain Secondary Antibody [Unconjugated] [NBP1-72788] - Lane 1: Human lambda (lambda chain). Lane 2: None. Load: 100 ng per lane. Primary antibody: Human lambda (lambda chain) Antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight800 Donkey anti-Goat at 1:20,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Other Band(s): None.
Immunocytochemistry/Immunofluorescence: Goat anti-Human Lambda Light Chain Secondary Antibody [Unconjugated] [NBP1-72788] - Human multiple myeloma cell lines 8226, LP-1, H929. 1. Cells immersion-fixed in 4% PFA for 10 minutes, washed 3x with PBS. Permeabilized with 0.5% Triton X-100 for 5 minutes, washed 3x with PBS. Blocked with 10% donkey serum 1 hour. NBP1-72788 antibody at 1:5000 O/N at 4C, washed 3x with PBS. Secondary antibody at 1:2000 donkey F(ab')Cy3 anti-goat IgG (H+L) 1 hour at room temp (Jackson #705-166-147); washed 3x with PBS. Imaging with Olympus/BIOVIEW Cy3 filter 300ms exposure. ICC/IF image submitted by a verified customer review.
Goat anti-Human Lambda Light Chain Secondary Antibody

Goat anti-Human Lambda Light Chain Secondary Antibody

Western Blot of Goat anti-Human lambda chain Antibody. Lane 1: Human lambda chain. Lane 2: None. Load: 100 ng per lane. Primary antibody: Human lambda chain Antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight800 Donkey anti-Goat at 1:20,000 for 30 min at RT. Block for 30 min at RT. Other Band(s): None.

Applications for Goat anti-Human Lambda Light Chain Secondary Antibody

Application
Recommended Usage

ELISA

1:4000 - 1:20000

Immunohistochemistry

1:1000 - 1:5000

Western Blot

1:500 - 1:2000
Application Notes
This product has been tested by ELISA and western blot and is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-72788 in the following applications:

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lambda Light Chain

All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). This antibody stains only lambda light chain containing cells in human lymphoid tissues.

Alternate Names

IGLC, immunoglobulin lambda constant group

Gene Symbol

IGLC1

Additional Lambda Light Chain Products

Product Documents for Goat anti-Human Lambda Light Chain Secondary Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Goat anti-Human Lambda Light Chain Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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