Simple Plex Human RAGE Cartridge

Name: Simple Plex Human RAGE Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000341

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000341

Simple Plex Human RAGE/AGER assay kit for use on Ella instrument. Contains cartridge, sample diluent SD06, and wash buffer.

Customize Your Simple Plex Cartridge

Create your custom Simple Plex cartridge for up to 8 targets. Choose from more than 250 targets across multiple species.

Build My Cartridge

Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.82 pg/mL
Assay Range:	12.4 - 7,570 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human RAGE Cartridge Summary:	Simple Plex assay for the detection of human Receptor for Advanced Glycation End product (RAGE) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human RAGE Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human RAGE Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human RAGE.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	19	19
Mean (pg/mL)	49.6	2301	45.3	2263
Standard Deviation	3.15	175	3.16	247
CV%	6.4	7.6	7	10.9

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=5)	108	106-110

Linearity

Samples containing and/or spiked with high concentrations of human RAGE were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human RAGE Cartridge

	Human RAGE Ella Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: RAGE/AGER

RAGE (Receptor for Advanced Glycation End product) is a transmembrane glycoprotein that binds advanced glycation end products (AGEs), beta-amyloid peptides, HMGB1/Amphoterin, and several S100 family proteins. AGEs are adducts formed by the non-enzymatic glycation and oxidation of proteins and lipids. A soluble form can also be generated by MMP-mediated shedding. RAGE is expressed in the CNS during development as well as in adult endothelial cells, smooth muscle cells, pericytes, monocytes, and neurons. It is locally upregulated in vascular inflammation (e.g. diabetes, atherosclerosis, vascular injury, Alzheimer’s disease). At these sites, RAGE binding to S100A1, EN-RAGE/S100A12, or S100B induces inflammatory immune cell adhesion and infiltration as well as vascular smooth muscle proliferation, neointimal expansion, atherosclerotic plaque development, and transport of A-beta into the cerebrospinal fluid. In cancer, RAGE binding to HMGB1, S100A8, or S100A9 promotes tumor growth and metastasis in addition to inflammatory cell infiltration.

To view our complete solutions for RAGE research, visit bio-techne.com.

Item	Value
Long Name	Receptor for Advanced Glycation End Products
Entrez Gene IDs	177 (Human); 11596 (Mouse); 81722 (Rat); 403168 (Canine)
Alternate Names	AGER, RAGE, RAGE isoform delta, RAGE isoform sRAGE-delta, Receptor for advanced glycosylation end products, SCARJ1, advanced glycosylation end product-specific receptor, receptor for advanced glycosylation end-products

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

Learn More

Product Documents for Simple Plex Human RAGE Cartridge

Product Datasheets

Download Product Insert

COA

Protocols & Manuals

Download Protocol