4-Thiouridine

Name: 4-Thiouridine
Brand: Tocris Bioscience
SKU: 7005

Tocris Bioscience, part of Bio-Techne | Catalog # 7005

Citations (9)

COA / SDS

Photoreactive uridine analog for RNA sequencing

Key Product Details

Description:	Photoreactive uridine analog for RNA sequencing
Chemical Name:	4-Thiouridine
Purity:	≥98% (HPLC)
Molecular Weight:	260.26

View Full Technical Details for 4-Thiouridine

Technical Data
Citations

Biological Activity for 4-Thiouridine

4-Thiouridine is a photoreactive uridine analog for RNA sequencing (RNA seq) and thiol(SH)-linked alkylation for the metabolic sequencing of RNA (SLAM seq). Used as a crosslinker to identify RNA binding proteins. Is incorporated into newly transcribed RNA and can be used to track RNA from synthesis to degradation.

Technical Data for 4-Thiouridine

M. Wt	260.26
Formula	C₉H₁₂N₂O₅S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	13957-31-8
PubChem ID	3032615
InChi Key	ZLOIGESWDJYCTF-XVFCMESISA-N
Smiles	OC[C@H]1O[C@@H](N2C=CC(NC2=O)=S)[C@@H]([C@@H]1O)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 4-Thiouridine

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	5.21	20
	DMSO	5.21	20

Preparing Stock Solutions for 4-Thiouridine

The following data is based on the product molecular weight 260.26.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.2 mM	19.21 mL	96.06 mL	192.12 mL
1 mM	3.84 mL	19.21 mL	38.42 mL
2 mM	1.92 mL	9.61 mL	19.21 mL
10 mM	0.38 mL	1.92 mL	3.84 mL

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Reconstitution Calculator

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References for 4-Thiouridine

References are publications that support the biological activity of the product.

Herzog Thiol-linked alkylation of RNA to assess expression dynamics. Nat.Methods 2017 PMID: 28945705
Schofield TimeLapse-seq: adding a temporal dimension to RNA sequencing through nucleoside recoding. Nat.Methods 2018 PMID: 29355846
Garibaldi Isolation of newly transcribed RNA using the metabolic label 4-thiouridine. Methods Mol.Biol. 2017 PMID: 28766297