AZ1 USP25/28 inhibitor

AZ1 USP25/28 inhibitor is a selective inhibitor of USP28 (IC₅₀ = 0.6 μM, K_d= 0.2 - 3.7 μM) and USP25 (IC₅₀ = 0.7 μM). AZ1 USP25/28 inhibitor exhibits selectivity for USP25 and USP28 over other DUB family members. The compound dose-dependently reduces c-Myc levels in colon carcinoma cells in vitro and induces cell death (EC₅₀ values are 18 - 20 μM). In a mouse Alzheimer's disease model, AZ1 USP25/28 inhibitor reduces amyloid burden, attenuates microglial activation and improves synaptic and cognitive function.