BRD PHOTAC-I-3

Name: BRD PHOTAC-I-3
Brand: Tocris Bioscience
SKU: 7319

Tocris Bioscience, part of Bio-Techne | Catalog # 7319

COA / SDS

Photoswitchable BET bromodomain Degrader (PHOTAC)

Key Product Details

Description:	Photoswitchable BET bromodomain Degrader (PHOTAC)
Chemical Name:	2-((S)-4-(4-Chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f]-[1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-N-(4-(2-(4-((E)-(2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-4-yl)diazenyl)-2,6-dimethoxyphenoxy)acetamido)butyl)acetamide
Purity:	≥98% (HPLC)
Molecular Weight:	935.45

View Full Technical Details for BRD PHOTAC-I-3

Technical Data
Citations

Biological Activity for BRD PHOTAC-I-3

BRD PHOTAC-I-3 is a photoswitchable BET bromodomain degrader (PHOTAC, PHOtochemically TArgeting Chimera). BRD PHOTAC-I-3 comprises a ligand for an E3 ligase, a photoswitch, and the BET bromodomain targeting ligand (+)-JQ1 (Cat. No. 4499). Exhibits cytotoxicity and degrades BRD4, BRD3 and to a lesser extent BRD2 in lymphoblast cells upon irradiation with 390 nm light, but not in the dark. BRD2 antibodies validated for Western Blot also available: Catalog # NBP1-30475 and NBP2-75422.

Licensing Information

Sold under license from New York University

Technical Data for BRD PHOTAC-I-3

M. Wt	935.45
Formula	C₄₆H₄₇ClN₁₀O₈S
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2370997-94-5
PubChem ID	154722038
InChi Key	SGZZPSOQAWRZGE-RCBZUBGNSA-N
Smiles	COC1=CC(/N=N/C2=C3CN(C(C3=CC=C2)=O)C4CCC(NC4=O)=O)=CC(OC)=C1OCC(NCCCCNC(C[C@@H]5N=C(C6=CC=C(C=C6)Cl)C7=C(N8C(C)=NN=C58)SC(C)=C7C)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for BRD PHOTAC-I-3

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	46.77	50

Preparing Stock Solutions for BRD PHOTAC-I-3

The following data is based on the product molecular weight 935.45.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	2.14 mL	10.69 mL	21.38 mL
2.5 mM	0.43 mL	2.14 mL	4.28 mL
5 mM	0.21 mL	1.07 mL	2.14 mL
25 mM	0.04 mL	0.21 mL	0.43 mL

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References for BRD PHOTAC-I-3

References are publications that support the biological activity of the product.

Reynders PHOTACs enable optical control of protein degradation. Sci.Adv. 2020 PMID: 32128406