CM 11

Name: CM 11
Brand: Tocris Bioscience
SKU: 6416

Tocris Bioscience, part of Bio-Techne | Catalog # 6416

COA / SDS

Homo-PROTAC® for self-degradation of pVHL30

Key Product Details

Description:	Homo-PROTAC^® for self-degradation of pVHL30
Chemical Name:	N¹,N²⁰-bis((S)-1-((2S,4R)-4-Hydroxy-2-((4-(4-methylthiazol-5-yl)benzyl)carbamoyl)pyrrolidin-1-yl)-3,3-dimethyl-1-oxobutan-2-yl)-3,6,9,12,15,18-hexaoxaicosanediamide
Purity:	≥98% (HPLC)
Molecular Weight:	1179.46

View Full Technical Details for CM 11

Technical Data
Citations

Biological Activity for CM 11

CM 11 is a PROTAC^® for self-degradation of the long form of VHL, pVHL30 (DC₅₀ < 100 nM). Composed of two VHL ligands joined by a linker. Exhibits > 1000-fold increased cellular activity compared to the parent VHL ligand, VH 032. Dimerizes pVHL30 with high avidity in vitro. Induced potent and rapid self-degradation of VHL in cell lines in a highly isoform dependent fashion. Negative control CMP 98 (Cat. No. 6417) also available.

PROTAC^® is a registered trademark of Arvinas Operations, Inc., and is used under license.

Licensing Information

Sold under licence from the University of Dundee

Technical Data for CM 11

M. Wt	1179.46
Formula	C₅₈H₈₂N₈O₁₄S₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2244684-49-7
PubChem ID	134160242
InChi Key	WGJCHHJGGFCCRS-DEYDLUNASA-N
Smiles	CC1=C(C2=CC=C(CNC([C@@H]3C[C@@H](O)CN3C([C@H](C(C)(C)C)NC(COCCOCCOCCOCCOCCOCC(N[C@@H](C(C)(C)C)C(N4[C@H](C(NCC5=CC=C(C6=C(C)N=CS6)C=C5)=O)C[C@@H](O)C4)=O)=O)=O)=O)=O)C=C2)SC=N1
E3 ligase	VHL
DC₅₀	10-100 nM - Degradation of pVHL30 in HeLa cells after 24 h treatment
Selectivity confirmed by global proteomics	No

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for CM 11

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	117.95	100

Preparing Stock Solutions for CM 11

The following data is based on the product molecular weight 1179.46.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	0.85 mL	4.24 mL	8.48 mL
5 mM	0.17 mL	0.85 mL	1.70 mL
10 mM	0.08 mL	0.42 mL	0.85 mL
50 mM	0.02 mL	0.08 mL	0.17 mL

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Reconstitution Calculator

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References for CM 11

References are publications that support the biological activity of the product.

Maniaci Homo-PROTACs: bivalent small-molecule dimerizers of the VHL E3 ubiquitin ligase to induce self-degradation. Nat.Commun. 2017 PMID: 29018234