Digoxigenin NHS ester

Name: Digoxigenin NHS ester
Brand: Tocris Bioscience
SKU: 7784

Tocris Bioscience, part of Bio-Techne | Catalog # 7784

COA / SDS

NHS ester hapten for labeling amino groups

Key Product Details

Description:	NHS ester hapten for labeling amino groups
Chemical Name:	(3β,5β,12β)-3-[2-[[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]amino]-2-oxoethoxy]-12,14-dihydroxycard-20(22)-enolide
Purity:	≥80% (HPLC)
Molecular Weight:	658.79

View Full Technical Details for Digoxigenin NHS ester

Technical Data
Citations

Biological Activity for Digoxigenin NHS ester

Digoxigenin NHS ester is an NHS ester hapten for labeling amino groups within biomolecules (oligonucleotides or proteins). Digoxigenin NHS ester is also used for in situ hybridization.

Technical Data for Digoxigenin NHS ester

M. Wt	658.79
Formula	C₃₅H₅₀N₂O₁₀
Storage	Store at -20°C
Purity	≥80% (HPLC)
CAS Number	129273-26-3
InChi Key	KHNDABJZSPPYLE-FUGFVFQCSA-N
Smiles	O[C@@]12[C@@]([C@@H](C(CO3)=CC3=O)CC1)([C@@H](C[C@@]4([H])[C@@]2([H])CC[C@@]5([H])[C@@]4(CC[C@@H](C5)OCC(NCCCCCC(ON6C(CCC6=O)=O)=O)=O)C)O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Digoxigenin NHS ester

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	65.88	100
	DMF	65.88	100

Preparing Stock Solutions for Digoxigenin NHS ester

The following data is based on the product molecular weight 658.79.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.52 mL	7.59 mL	15.18 mL
5 mM	0.30 mL	1.52 mL	3.04 mL
10 mM	0.15 mL	0.76 mL	1.52 mL
50 mM	0.03 mL	0.15 mL	0.30 mL

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Reconstitution Calculator

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References for Digoxigenin NHS ester

References are publications that support the biological activity of the product.

Heyduk Peptide ligand-based ELISA reagents for antibody detection. Anal.Biochem. 2018 PMID: 30130491