Indibulin

Name: Indibulin
Brand: Tocris Bioscience
SKU: 3728

Tocris Bioscience, part of Bio-Techne | Catalog # 3728

Citations (2)

COA / SDS

Microtubule destabilizer

Key Product Details

Description:	Microtubule destabilizer
Alternative Names:	D 24851
Chemical Name:	2-[1-(4-Chlorobenzyl)-1H-indol-3-yl]-2-oxo-N-pyridin-4-ylacetamide
Purity:	≥98% (HPLC)
Molecular Weight:	389.83

View Full Technical Details for Indibulin

Technical Data
Citations

Biological Activity for Indibulin

Indibulin is a microtubule destabilizer that blocks tubulin polymerization (IC₅₀ = 0.3 μM). Blocks cell cycle progression at metaphase and displays antitumor activity against a variety of malignancies in vitro (IC₅₀ values ranging from 0.036 - 0.285 μM). Induces complete tumor remission in the Yoshida AH13 rat sarcoma model in vivo. Lacks neurotoxicity due to an ability to discriminate between posttranslationally modified tubulin in mature neuronal microtubules and less-modified tubulin present in immature neuronal or nonneuronal microtubules. Displays oral availability and efficacy towards MDR tumor cells.

Technical Data for Indibulin

M. Wt	389.83
Formula	C₂₂H₁₆ClN₃O₂
Storage	Desiccate at -20°C
Purity	≥98% (HPLC)
CAS Number	204205-90-3
PubChem ID	2929
InChi Key	SOLIIYNRSAWTSQ-UHFFFAOYSA-N
Smiles	ClC(C=C3)=CC=C3CN2C1=CC=CC=C1C(C(C(NC4=CC=NC=C4)=O)=O)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Indibulin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	9.75	25

Preparing Stock Solutions for Indibulin

The following data is based on the product molecular weight 389.83.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.25 mM	10.26 mL	51.30 mL	102.61 mL
1.25 mM	2.05 mL	10.26 mL	20.52 mL
2.5 mM	1.03 mL	5.13 mL	10.26 mL
12.5 mM	0.21 mL	1.03 mL	2.05 mL

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References for Indibulin

References are publications that support the biological activity of the product.

Kanzawa Microtubule inhibitor D-24851 induces p53-independent apoptotic cell death in malignant glioma cells through Bcl-2 phosphorylation and Bax translocation. Int.J.Oncol. 2005 PMID: 15703812
Wienecke and Bacher Indibulin, a novel microtubule inhibitor, discriminates between mature and nonneuronal tubulin. Cancer Res. 2009 PMID: 19118000
Bacher D-24851, a novel synthetic microtubule inhibitor, exerts curative antitumoral activity in vivo, shows efficacy toward multidrug-resistant tumor cells, and lacks neurotoxicity. Cancer Res. 2001 PMID: 11196193