LIMKi 3

Name: LIMKi 3
Brand: Tocris Bioscience
SKU: 4745

Tocris Bioscience, part of Bio-Techne | Catalog # 4745

Citations (22)

COA / SDS

Potent LIM kinase inhibitor; antitumor

Key Product Details

Description:	Potent LIM kinase inhibitor; antitumor
Alternative Names:	BMS 5
Chemical Name:	N-[5-[1-(2,6-Dichlorophenyl)-3-(difluoromethyl)-1H-pyrazol-5-yl]-2-thiazolyl]-2-methylpropanamide
Purity:	≥98% (HPLC)
Molecular Weight:	431.29

View Full Technical Details for LIMKi 3

Technical Data
Citations

Biological Activity for LIMKi 3

LIMKi 3 is a potent LIM kinase inhibitor (IC₅₀ values are 7 and 8 nM for LIMK1 and LIMK2 respectively). Inhibits cofilin phosphorylation in MDA-MB-231 breast cancer cells. Reduces MDA-MB-231 tumor cell invasion in a 3D matrigel invasion assay.

Compound Libraries for LIMKi 3

LIMKi 3 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Kinase Inhibitor Library. Find out more about compound libraries available from Tocris.

Technical Data for LIMKi 3

M. Wt	431.29
Formula	C₁₇H₁₄Cl₂F₂N₄OS
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	1338247-35-0
PubChem ID	56965901
InChi Key	IVUGBSGLHRJSSP-UHFFFAOYSA-N
Smiles	FC(F)C1=NN(C2=C(Cl)C=CC=C2Cl)C(C3=CN=C(NC(C(C)C)=O)S3)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for LIMKi 3

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	43.13	100

Preparing Stock Solutions for LIMKi 3

The following data is based on the product molecular weight 431.29.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.32 mL	11.59 mL	23.19 mL
5 mM	0.46 mL	2.32 mL	4.64 mL
10 mM	0.23 mL	1.16 mL	2.32 mL
50 mM	0.05 mL	0.23 mL	0.46 mL

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References for LIMKi 3

References are publications that support the biological activity of the product.

Scott LIM kinases are required for invasive path generation by tumor and tumor-associated stromal cells. J.Cell Biol. 2010 PMID: 20876278
Sparrow The actin-severing protein cofilin is downstream of neuregulin signaling and is essential for Schwann cell myelination. J.Neurosci. 2012 PMID: 22496574
Ross-Macdonald Identification of a nonkinase target mediating cytotoxicity of novel kinase inhibitors. Mol.Cancer Ther. 2008 PMID: 19001433