ML 221

Tocris Bioscience, part of Bio-Techne | Catalog # 4748

COA / SDS

Apelin antagonist

Key Product Details

Description:	Apelin antagonist
Chemical Name:	5-[(4-Nitrobenzoyl)oxy]-2-[(2-pyrimidinylthio)methyl]-4H-pyran-4-one
Purity:	≥99% (HPLC)
Molecular Weight:	385.35

View Full Technical Details for ML 221

Technical Data
Citations

Biological Activity for ML 221

ML 221 is an apelin receptor (APJ) antagonist (IC₅₀ values are 0.70 and 1.75 μM in a cAMP assay and β-arrestin assay, respectively). Displays >37-fold selectivity over the closely related angiotensin II type 1 (AT₁) receptor. Exhibits no toxicity towards human hepatocytes at concentrations >50 μM.

Technical Data for ML 221

M. Wt	385.35
Formula	C₁₇H₁₁N₃O₆S
Storage	Store at +4°C
Purity	≥99% (HPLC)
CAS Number	877636-42-5
PubChem ID	7217941
InChi Key	UASIRTUMPRQVFY-UHFFFAOYSA-N
Smiles	O=C(C3=CC=C([N+]([O-])=O)C=C3)OC1=COC(CSC2=NC=CC=N2)=CC1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for ML 221

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	3.85	10

Preparing Stock Solutions for ML 221

The following data is based on the product molecular weight 385.35.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	25.95 mL	129.75 mL	259.50 mL
0.5 mM	5.19 mL	25.95 mL	51.90 mL
1 mM	2.60 mL	12.98 mL	25.95 mL
5 mM	0.52 mL	2.60 mL	5.19 mL

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References for ML 221

References are publications that support the biological activity of the product.

Maloney Discovery of 4-oxo-6-((pyrimidin-2-ylthio)methyl)-4H-pyran-3-yl 4-nitrobenzoate (ML221) as a functional antagonist of the apelin (APJ) receptor. Bioorg.Med.Chem.Lett. 2012 PMID: 23010269