ML 335

Name: ML 335
Brand: Tocris Bioscience
SKU: 6887

Tocris Bioscience, part of Bio-Techne | Catalog # 6887

Citations (3)

COA / SDS

Selective K2P2.1 (TREK-1) and K2P10.1 (TREK-2) activator

Key Product Details

Description:	Selective K_2P2.1 (TREK-1) and K_2P10.1 (TREK-2) activator
Chemical Name:	N-[(2,4-Dichlorophenyl)methyl]-4-[(methylsulfonyl)amino]benzamide
Purity:	≥98% (HPLC)
Molecular Weight:	373.25

View Full Technical Details for ML 335

Technical Data
Citations

Biological Activity for ML 335

ML 335 is a selective K_2P2.1 (TREK-1; KCNK2) and K_2P10.1 (TREK-2; KCNK10) activator (EC₅₀ values of 14.3 μM and 5.2 μM, respectively); directly stimulates the C-type gate via the K_2P modulator pocket. Does not activate K_2P4.1 (TRAAK).

Technical Data for ML 335

M. Wt	373.25
Formula	C₁₅H₁₄Cl₂N₂O₃S
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	825658-06-8
PubChem ID	1243054
InChi Key	RDFIQTZRJRVFHK-UHFFFAOYSA-N
Smiles	CS(=O)(NC1=CC=C(C(NCC2=C(C=C(C=C2)Cl)Cl)=O)C=C1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for ML 335

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	37.33	100
	ethanol	7.46	20

Preparing Stock Solutions for ML 335

The following data is based on the product molecular weight 373.25.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.68 mL	13.40 mL	26.79 mL
5 mM	0.54 mL	2.68 mL	5.36 mL
10 mM	0.27 mL	1.34 mL	2.68 mL
50 mM	0.05 mL	0.27 mL	0.54 mL

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References for ML 335

References are publications that support the biological activity of the product.

Schewe A pharmacological master key mechanism that unlocks the selectivity filter gate in K⁺ channels. Science 2019 PMID: 30792303
Gada Two-pore domain potassium channels: emerging targets for novel analgesic drugs: IUPHAR Review 26. Br.J.Pharmacol. 2019 PMID: 30325008
Lolicato K_2P2.1 (TREK-1)-activator complexes reveal a cryptic selectivity filter binding site. Nature 2017 PMID: 28693035
Pope Protein and chemical determinants of BL-1249 action and selectivity for K_2P channels. ACS Chem.Neurosci. 2018 PMID: 30089357