SCR7 pyrazine

Name: SCR7 pyrazine
Brand: Tocris Bioscience
SKU: 5342

Tocris Bioscience, part of Bio-Techne | Catalog # 5342

Citations (4)

COA / SDS

Enhances HDR efficiency

Key Product Details

Description:	Enhances HDR efficiency
Chemical Name:	2,3-Dihydro-6,7-diphenyl-2-thioxo-4(1H)-pteridinone
Purity:	≥98% (HPLC)
Molecular Weight:	332.38

View Full Technical Details for SCR7 pyrazine

Technical Data
Citations

Biological Activity for SCR7 pyrazine

SCR7 pyrazine enhances CRISPR-Cas9-mediated homology-directed repair (HDR) efficiency in vitro up to 19-fold. Inhibits nonhomologous end-joining (NHEJ).

We have discovered that the commercially available SCR7 material supplied by other vendors does not match the chemical structure that it is being sold under. Through detailed chemical analysis carried out by our Analytical Quality Control experts, we believe that the commercially available SCR7 material, including that used in recent publications to enhance CRISPR efficiency, is in fact an analog of SCR7 that we have named 'SCR7 pyrazine'.

View more information regarding the structure of SCR7.

Technical Data for SCR7 pyrazine

M. Wt	332.38
Formula	C₁₈H₁₂N₄OS
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	14892-97-8
PubChem ID	10688007
InChi Key	GSRTWXVBHGOUBU-UHFFFAOYSA-N
Smiles	O=C(C(N=C(C3=CC=CC=C3)C(C4=CC=CC=C4)=N2)=C2N1)NC1=S

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SCR7 pyrazine

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	33.44	100
	ethanol	6.69	20

Preparing Stock Solutions for SCR7 pyrazine

The following data is based on the product molecular weight 332.38.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.01 mL	15.04 mL	30.09 mL
5 mM	0.60 mL	3.01 mL	6.02 mL
10 mM	0.30 mL	1.50 mL	3.01 mL
50 mM	0.06 mL	0.30 mL	0.60 mL

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References for SCR7 pyrazine

References are publications that support the biological activity of the product.

Greco SCR7 is neither a selective nor a potent inhibitor of human DNA ligase IV. DNA Repair (Amst) 2016 PMID: 27235626
Maruyama Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining. Nat.Biotechnol. 2015 PMID: 25798939
Greco Synthesis and structure determination of SCR7, a DNA ligase inhibitor Teterahedron Lett.
Chu Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat.Biotechnol. 2015 PMID: 25803306