SPP 86

Name: SPP 86
Brand: Tocris Bioscience
SKU: 5391

Tocris Bioscience, part of Bio-Techne | Catalog # 5391

Citations (3)

COA / SDS

Potent RET inhibitor

Key Product Details

Description:	Potent RET inhibitor
Chemical Name:	1-(1-Methylethyl)-3-(2-phenylethynyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine
Purity:	≥98% (HPLC)
Molecular Weight:	277.32

View Full Technical Details for SPP 86

Technical Data
Citations

Biological Activity for SPP 86

SPP 86 is a potent RET inhibitor (IC₅₀ = 8 nM). Also exhibits inhibitory activity at EphA1, FGFR1, Flt4, Lck and Yes. Inhibits proliferation of HCT 116 and TPC1 cells in vitro. Cell permeable.

Compound Libraries for SPP 86

SPP 86 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for SPP 86

M. Wt	277.32
Formula	C₁₆H₁₅N₅
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	1357349-91-7
PubChem ID	66549796
InChi Key	JQOIRTDBHMDWMT-UHFFFAOYSA-N
Smiles	CC(C)N1N=C(C#CC3=CC=CC=C3)C2=C1N=CN=C2N

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for SPP 86

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	27.73	100
	ethanol	5.55	20

Preparing Stock Solutions for SPP 86

The following data is based on the product molecular weight 277.32.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.61 mL	18.03 mL	36.06 mL
5 mM	0.72 mL	3.61 mL	7.21 mL
10 mM	0.36 mL	1.80 mL	3.61 mL
50 mM	0.07 mL	0.36 mL	0.72 mL

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References for SPP 86

References are publications that support the biological activity of the product.

Klein Design, synthesis and characterization of a highly effective inhibitor for analog-sensitive (as) kinases. PLoS ONE 2011 PMID: 21698101
Dinér Preparation of 3-substituted-1-isopropyl-1H-pyrazolo[3,4-d]pyrimidin-4-amines as RET kinase inhibitors. J.Med.Chem. 2012 PMID: 22559926