WZ 4003

Name: WZ 4003
Brand: Tocris Bioscience
SKU: 5177

Tocris Bioscience, part of Bio-Techne | Catalog # 5177

Citations (9)

COA / SDS

Potent and selective NUAK1/2 inhibitor

Key Product Details

Description:	Potent and selective NUAK1/2 inhibitor
Chemical Name:	N-3-[[5-Chloro-2-[[2-methoxy-4-(4-methyl-1-piperazinyl)phenyl]amino]-4-pyrimidinyl]oxy]phenyl]propanamide
Purity:	≥98% (HPLC)
Molecular Weight:	496.99

View Full Technical Details for WZ 4003

Technical Data
Citations

Biological Activity for WZ 4003

WZ 4003 is a potent and selective NUAK1/2 inhibitor (IC₅₀ values are 20 and 100 nM respectively). Exhibits no significant inhibition against a panel of 139 kinases, including ten AMPK family members. Inhibits NUAK1-mediated MYPT1 phosphorylation. Also inhibits cell proliferation in U2OS cells.

External Portal Information

Chemicalprobes.org is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of WZ 4003 is reviewed on the chemical probes website.

Technical Data for WZ 4003

M. Wt	496.99
Formula	C₂₅H₂₉ClN₆O₃
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1214265-58-3
PubChem ID	72200024
InChi Key	SDGJBAUIGHSMRI-UHFFFAOYSA-N
Smiles	CN(CC3)CCN3C(C=C2)=CC(OC)=C2NC1=NC=C(Cl)C(OC4=CC(NC(CC)=O)=CC=C4)=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for WZ 4003

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	1eq. HCl	24.85	50
	DMSO	9.94	20 with gentle warming

Preparing Stock Solutions for WZ 4003

The following data is based on the product molecular weight 496.99.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	4.02 mL	20.12 mL	40.24 mL
2.5 mM	0.80 mL	4.02 mL	8.05 mL
5 mM	0.40 mL	2.01 mL	4.02 mL
25 mM	0.08 mL	0.40 mL	0.80 mL

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References for WZ 4003

References are publications that support the biological activity of the product.

Banerjee Interplay between Polo kinase, LKB1-activated NUAK1 kinase, PP1βMYPT1 phosphatase complex and the SCFβTrCP E3 ubiquitin ligase. Biochem.J. 2014 PMID: 24785407
Banerjee Characterization of WZ4003 and HTH-01-015 as selective inhibitors of the LKB1-tumour-suppressor-activated NUAK kinases. Biochem.J. 2014 PMID: 24171924
Zhou Novel mutant-selective EGFR kinase inhibitors against EGFR T790M. Nature 2009 PMID: 20033049