ImmPACT (TM) DAB HRP Substrate

Novus Biologicals, part of Bio-Techne | Catalog # SK-4105-NB

Novus Biologicals, part of Bio-Techne
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Key Product Details

Applications

ELISA, Functional Assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Product Summary for ImmPACT (TM) DAB HRP Substrate

Features:
2-4 fold greater sensitivity than original substrate kit Optimized for IHC/ICC/ISH and blots Heat Stable Permanent mounting Ideal for single and multiple labeling Working solutions stable for up to 14 days at 4C One year expiry date Supplied as 120 ml of diluent and concentrated DAB stock solution

Product Specifications

Application Notes

This product is an entirely new formula for a diaminobenzidine based peroxidase substrate. ImmPACT(TM) DAB produces a dark brown reaction product that is crisper and is 3-4 times more sensitive than the original Vector DAB substrate kit. It can be used for both manual and automated staining methods. Sections stained with DAB can also be viewed by darkfield and electron microscopy. Slides developed with DAB can be dehydrated, cleared, and permanently mounted. The ImmPACT(TM) DAB working solution is stable for at least five days at room temperature and at least two weeks when stored at 4C. This product consists of 120 ml of diluent and a convenient concentrated stock solution of highly purified DAB. This product can also be used on blots..

Kit Contents for ImmPACT (TM) DAB HRP Substrate

  • ImmPACT™ DAB Chromogen concentrate (3.6 mL)
  • ImmPACT™ DAB Diluent (120 mL)

Formulation, Preparation, and Storage

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C in the dark.

Background: ImmPACT (TM) DAB HRP Substrate

DAB (3,3-diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications. DAB chromogen is effective as a single label or as a second color for multiple antigen labeling. Because of its heat-resistance, DAB can be used in IHC/ ISH double labeling applications. With the aid of imaging systems and software, the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co-localized.

Additional ImmPACT (TM) DAB HRP Substrate Products

Product Documents for ImmPACT (TM) DAB HRP Substrate

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ImmPACT (TM) DAB HRP Substrate

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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