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VECTASHIELD(R) Antifade Mounting Medium with Propidium Iodide

Novus Biologicals, part of Bio-Techne | Catalog # H-1300-NB

Novus Biologicals, part of Bio-Techne
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H-1300-NB

Key Product Details

Applications

Functional Assay, Immunocytochemistry/ Immunofluorescence, Microscopy

Product Summary for VECTASHIELD(R) Antifade Mounting Medium with Propidium Iodide

VECTASHIELD Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. VECTASHIELD Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes.

Features:

Inhibits photobleaching of fluorescent dyes and fluorescent proteins Ideal refractive index (1.45) Ready-to-use No warming necessary Can be stored without sealing for long term analysis Non-hardening formulation Propidium iodide (PI) counterstain

The original VECTASHIELD Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4C, protected from light.

VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. The original VECTASHIELD Mounting Medium is available with or without the counterstain PI (propidium iodide). PI is a reddish nuclear counterstain with a broad excitation range at around 530 nm and emission at about 615 nm.

Product Specifications

Application Notes

Refractive index is 1.45..

Scientific Data Images for VECTASHIELD(R) Antifade Mounting Medium with Propidium Iodide

Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Antifade Mounting Medium with Propidium Iodide [H-1300-NB]

Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Antifade Mounting Medium with Propidium Iodide [H-1300-NB]

Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Antifade Mounting Medium with Propidium Iodide [H-1300-NB] - FastTag Fluorescein labeled pUC1.77 detected with Biotinylated Anti-Fluorescein and Fluorescein Avidin DCS, and counterstained with VECTASHIELD with PI.
In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with Propidium Iodide [H-1300-NB]

In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with Propidium Iodide [H-1300-NB]

In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with Propidium Iodide [H-1300-NB] - Mounting Medium with Propidium Iodide [H-1300-NB] - Human chromosome 6 paint detected with FITC-labeled probe (yellow) and mounted with VECTASHIELD Mounting Medium with PI (red) Photo courtesy of Cytocell Ltd.

Formulation, Preparation, and Storage

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C in the dark.

Background: VECTASHIELD(R) Antifade Mounting Medium with Propidium Iodide

Additional VECTASHIELD(R) Antifade Mounting Medium with Propidium Iodide Products

Product Documents for VECTASHIELD(R) Antifade Mounting Medium with Propidium Iodide

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VECTASHIELD(R) Antifade Mounting Medium with Propidium Iodide

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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