VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium with Phalloidin

VECTASHIELD Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. VECTASHIELD Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes.

Features:

Inhibits photobleaching of fluorescent dyes and fluorescent proteins Ideal refractive index (1.45) Ready-to-use - from the refrigerator to the benchtop No warming necessary Can be stored without sealing for long term analysis Hardening formulation TRITC-Phalloidin counterstain
VECTASHIELD HardSet Mounting Medium preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved.

VECTASHIELD HardSet Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. This special formulation of VECTASHIELD HardSet Mounting Medium contains TRITC-phalloidin. Phalloidin is a bicyclic heptapeptide that specifically binds at the interface between the F-actin subunits. Fluorescent derivatives of phalloidin are used to stain actin filaments.

TRITC (tetramethylrhodamine) is excited at 544 nm and emits at 572 nm, producing an orange-red fluorescence.