AbK

Name: AbK
Brand: Tocris Bioscience
SKU: 5113

Tocris Bioscience, part of Bio-Techne | Catalog # 5113

Citations (2)

COA / SDS

Photo-crosslinking probe; unnatural amino acid

Key Product Details

Description:	Photo-crosslinking probe; unnatural amino acid
Chemical Name:	N⁶-[[2-(3-Methyl-3H-diazirin-3-yl)ethoxy]carbonyl]-L-lysine
Purity:	≥98% (HPLC)
Molecular Weight:	272.3

View Full Technical Details for AbK

Technical Data
Citations

Biological Activity for AbK

AbK is an unnatural amino acid. Acts as a UV light-activated (369 nm) photocrosslinking probe when incorporated into proteins by pyrrolysyl tRNA synthetase/tRNA_CUA pairs.

Technical Data for AbK

M. Wt	272.3
Formula	C₁₁H₂₀N₄O₄.CF₃CO₂H
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1253643-88-7
PubChem ID	66825403
InChi Key	LUCMNTLJAFTFDU-QMMMGPOBSA-N
Smiles	N[C@H](C(O)=O)CCCCNC(OCCC1(C)N=N1)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for AbK

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	2.72	10 with gentle warming
	1eq. HCl	27.23	100

Preparing Stock Solutions for AbK

The following data is based on the product molecular weight 272.3.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.67 mL	18.36 mL	36.72 mL
5 mM	0.73 mL	3.67 mL	7.34 mL
10 mM	0.37 mL	1.84 mL	3.67 mL
50 mM	0.07 mL	0.37 mL	0.73 mL

Molarity Calculator

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Reconstitution Calculator

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References for AbK

References are publications that support the biological activity of the product.

Kleiner A chemical proteomics approach to reveal direct protein-protein interactions in living cells. Cell Chem.Biol. 2018 PMID: 29104064
Chatterjee Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells. Proc.Natl.Acad.Sci.U.S.A. 2013 PMID: 23818609
Lowder Visualizing protein partnerships in living cells and organisms. Curr.Opin.Cell Biol. 2011 PMID: 22104179
Ai Probing protein-protein interactions with a genetically encoded photo-crosslinking amino acid. Chembiochem 2011 PMID: 21678540

AbK