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AFM primer

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-71653

Novus Biologicals, part of Bio-Techne
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NBP1-71653
NBP1-71653SS

Key Product Details

Applications

Chromatin Immunoprecipitation

Product Specifications

Applications

Chromatin Immunoprecipitation (ChIP) (0.5 uM)

Application Notes

This AFM primer is useful for PCR in chromatin precipitation. Amplicon size for this primer set is 101 base pairs.

Reactivity Notes

Human.

Scientific Data Images for AFM primer

Chromatin Immunoprecipitation: AFM primer [NBP1-71653] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.
Chromatin Immunoprecipitation: AFM primer [NBP1-71653] - 2 ug of NB21-1072 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).

Formulation, Preparation, and Storage

Formulation

10 uM in TE buffer.

Preservative

No Preservative

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AFM primer

Primers are designed to amplify DNA in intron 1 of the human AFM gene locus. AFM is expressed in the liver and therefore silent in most cell types. AFM is associated with modified histones targeted to repressed genes.

Additional AFM primer Products

Product Documents for AFM primer

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AFM primer

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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