CpG oligodeoxynucleotides
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26237
Key Product Details
Species
Mouse
Applications
Functional Assay
Product Summary for CpG oligodeoxynucleotides
Mouse Sequence CpG ODN (1585) Type A: 5' G*GGGTCAACGTTGAG*G*G*G*G*G 3'
Human/Mouse Sequence CpG ODN (2216) Type A: 5' G*GGGGACGATCGTCG*G*G*G*G*G 3'
Human Sequence CpG ODN (2006) Type B: 5' T*C*G*T*C*G*T*T*T*T*G*T*C*G*T*T*T*T*G*T*C*G*T*T 3'
Mouse Sequence CpG ODN (1826) Type B: 5' T*C*C*A*T*G*A*C*G*T*T*C*C*T*G*A*C*G*T*T 3’
Mouse Sequence CpG ODN(1668) Type B: 5' T*C*C*A*T*G*A*C*G*T*T*C*C*T*G*A*T*G*C*T 3'
Mouse Sequence CpG ODN (1018) Type B: 5' T*G*A*C*T*G*T*G*A*A*C*G*T*T*C*G*A*G*A*T*G*A 3'
Human/Mouse Sequence CpG ODN (C274) Type C: 5' T*C*G*T*C*G*A*A*C*G*T*T*C*G*A*G*A*T*G*A*T 3'
Human/Mouse Sequence CpG ODN (C695) Type C: 5' T*C*G*A*A*C*G*T*T*C*G*A*A*C*G*T*T*C*G*A*A*C*G*T*T 3'
Human/Mouse Sequence CpG ODN (C792) Type C: 5' T*C*G*A*A*C*G*T*T*C*G*A*A*C*G*T*T*C*G*A*A*C*G*T*T*C*G*A*A*T 3'
(* Indicates a phosphorothioate modification)
Functionality: This product is useful for the activation of TLR9 and the stimulation of TLR9 has been reported with 5-20 ug/ml.
Product Specifications
Applications
Ligand Activation (5 - 20 ug/ml)
Application Notes
A TLR9/NF-kB SEAP reporter construct in a HEK 293 cell line was used as a model system for studying hTLR9 activation.
Scientific Data Images for CpG oligodeoxynucleotides
CpG oligodeoxynucleotides [NBP2-26237]
CpG oligodeoxynucleotides [NBP2-26237] - Evaluation of various CpG ODN effects on activation of human TLR9. TLR9/NF-kB/SEAPorter HEK 293 cells were plated in 96-well plates at 7.5 x 104 cells/well for 16 h. Cells were treated with various CpG ODNs at different concentrations (1, 5, 10 and 20 ug/ml) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit. Note: Both negative hCpG and mCpG are the negative control ODNs.
CpG oligodeoxynucleotides [NBP2-26237] - Evaluation of various CpG ODN effects on activation of mouse TLR9. NF-kB/SEAPorte RAW cells were plated in 96-well plates at 7.5 x 10^4 cells/ well for 16 h. Cells were treated with various CpG ODNs at different concentrations (1, 5, 10 and 20 ug/ml) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit.RAW264.7 cells endogenously express functional TLR9, highly responding to CpG ODNs. Both negative hCpG and mCpG are the negative control ODNs.
Kit Contents for CpG oligodeoxynucleotides
- NBP2-31132SS: 25 ug mouse CpG Type B ODN (1826)
- NBP2-31134SS: 25 ug human CpG Type B ODN (2006)
- NBP2-31136SS: 25 ug human CpG Type A ODN (2216)
- NBP2-31137SS: 25 ug human CpG Type C ODN (C274)
- NBP2-31138SS: 25 ug human CpG Type C ODN (C695)
- NBP2-31139SS: 25 ug mouse CpG Type C ODN (C792)
- NBP2-31140SS: 25 ug mouse CpG Type A ODN (1585)
- NBP2-31141SS: 25 ug mouse CpG Type B ODN (1668)
- NBP2-31142SS: 25 ug mouse CpG Type B ODN (1018)
Formulation, Preparation, and Storage
Formulation
Sterile water
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CpG oligodeoxynucleotides
Alternate Names
CpG ODN, CpG ODN (2006), oligodeoxynucleotides (ODN)
Additional CpG oligodeoxynucleotides Products
Product Documents for CpG oligodeoxynucleotides
Product Specific Notices for CpG oligodeoxynucleotides
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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