Fluoromount

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Aqueous mounting medium for fluorescent staining

This product is compatible with FITC, phycoerythrin, phycocyanin and allophycocyanin. It can also be used with other fluorescent markers such as Texas Red and rhodamine.

Protocol Recommendations
1. Mounting Procedure: Remove red tip from the bottle and cut thetip off with a sharp razor or scissors. This will help achieve asmooth flow of Fluoromount and prevent formation of tinybubbles.
2. Place the bottle upside down in a container before use. This will also help clear Fluoromount of bubbles.
3. Blot excess water from the slide without letting tissue specimendry. Make sure tissue is wet prior to mounting.
4. Apply 2-3 drops of Fluoromount on the tissue section. Gentlyrotate the slide to make a thin. Make sure the tissue is completely covered.
5. Allow Fluoromount to dry for 30-45 minutes at room temperature.
6. Seal coverslip edges with nail polish for long term storage.
7. Removal of Fluoromount: Fluoromount is an aqueous-based mounting medium that can easily be removed by soaking theslides in de-ionized water.
8. Place the slide in a beaker full of de-ionized water on a magnetic stirrer.
9. Leave the slide for few hours or overnight with gentle stirring for complete removal of dry Fluoromount.

0.05% Sodium Azide

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at room temperature.