INSTA-Blot Mouse Tissues
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30111
Key Product Details
Applications
SDS-PAGE
Product Summary for INSTA-Blot Mouse Tissues
Ready-to-use PVDF membrane with 11 mouse tissue lysates (20 ug total protein per lane).
INSTA-Blot͐ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot͐ Mouse Tissues NBP2-30111 is a simple and fast solution for screening proteins from various mouse tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-Blot͐,immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
Product Specifications
Application Notes
Tissues are from Balb/c mice. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail(PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20%glycerol, 0.4% B-mercaptoethanol).
Scientific Data Images for INSTA-Blot Mouse Tissues
![SDS-PAGE: INSTA-Blot Mouse Tissues [NBP2-30111]](https://resources.bio-techne.com/images/products/INSTA-Blot-Mouse-Tissues-SDS-Page-NBP2-30111-img0001.jpg "SDS-PAGE: INSTA-Blot Mouse Tissues [NBP2-30111]")
SDS-PAGE: INSTA-Blot Mouse Tissues [NBP2-30111]
SDS-Page: INSTA-Blot Mouse Tissues [NBP2-30111] - Mouse Tissues. Approximately 20 ug per lane of different mouse tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker (Mark12); 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. TestisFormulation, Preparation, and Storage
Preparation Method
INSTA-Blot™ Mouse Tissues NBP2-30111contains denatured proteins from mouse lysates loaded at 20 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blottedfor 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried andpackaged under anoxic conditions. The INSTA-Blot͐ is sold ready-to-use in your western blot protocol.
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at room temperature.
Background: INSTA-Blot Mouse Tissues
Alternate Names
INSTA Blot Mouse Tissues, INSTA-Blot Mouse, INSTABlot Mouse Tissues
Additional INSTA-Blot Mouse Tissues Products
Product Documents for INSTA-Blot Mouse Tissues
Product Specific Notices for INSTA-Blot Mouse Tissues
Mark12 is used as the molecular weight marker for this product.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.
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