ND OLLAS/DYKDDDDK Epitope Tag Expression and Detection Kit

Novus Biologicals, part of Bio-Techne | Catalog # NBK1-19033

Novus Biologicals, part of Bio-Techne
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NBK1-19033
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Key Product Details

Species

Non-species specific

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Kit Type

Expression and Detection Kit

Scientific Data Images for ND OLLAS/DYKDDDDK Epitope Tag Expression and Detection Kit

Western Blot: ND OLLAS/DYKDDDDK Epitope Tag Expression and Detection Kit [NBK1-19033]

Western Blot: ND OLLAS/DYKDDDDK Epitope Tag Expression and Detection Kit [NBK1-19033]

Western Blot: ND OLLAS/DYKDDDDK Epitope Tag Kit [NBK1-19033] - Comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies OLLAS NBP1-06713, anti-DYKDDDDK NBP1-06712 and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG TM and ANTI-FLAG TM are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
Other: ND OLLAS/DYKDDDDK Epitope Tag Expression and Detection Kit [NBK1-19033]

Other: ND OLLAS/DYKDDDDK Epitope Tag Expression and Detection Kit [NBK1-19033]

ND OLLAS/DYKDDDDK Epitope Tag Kit [NBK1-19033] - Vector Map of pCMV-SD OLLAS

Kit Contents for ND OLLAS/DYKDDDDK Epitope Tag Expression and Detection Kit

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C. Do not freeze.

Background: ND OLLAS/DYKDDDDK Epitope Tag

The pCMV-N-DYK-OLLAS vector provides a convenient vector to express recombinant proteins under control of the CMV promoter with both an N-terminal OLLAS tag and a DYKDDDDK tag. Using this vector the protein will be expressed in the cytoplasm. We recommend inserting your protein of interest into the vector in frame using the Xba I and EcoR I/Not I restriction sites shown above. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants. The OLLAS tag has been shown to be detectable at ~100-fold lower levels that similar commercially available tags using the OLLA-2 monoclonal antibody. The OLLAS tag has also been demonstrated to provide superior functionality whether used as an N-terminal, C-terminal, or internal tag on recombinant and fusion proteins without interfering with the biological activity of the tagged protein.

Alternate Names

OLLA-2

Additional ND OLLAS/DYKDDDDK Epitope Tag Products

Product Documents for ND OLLAS/DYKDDDDK Epitope Tag Expression and Detection Kit

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ND OLLAS/DYKDDDDK Epitope Tag Expression and Detection Kit

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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