I am having a retroviral system in place which allows me to infect mouse cell lines. The vectors I have are MIG.R (with my gene of interest), pCL-ECO and VSV-g. I know that my pCL-ECO vector is only suitable for infecting mouse cells. My question is: Is it possible to use my MIG.R and VSV-g vectors, and exchange the mouse specific pCL-ECO with pCL-Ampho or pCL-10A1 packaging vector which you both offer for purchase (Cat Numbers are 10045P, 10046P, 10047P). My aim is to infect human cell lines with my gene of interest.

These vectors, while also sold separately, are components of the RetroMax system 10040K. The vectors are designed to be used as described in the 10040K manual. Yes, you are correct, pCL-ECO is for mouse and rat, and not for human. We have not evaluated the Retromax system with the MIG.R and VSV-g vectors, and can not promise they would be compatible with your vectors. The Retromax packaging vectors are designed to be used with the expression vectors in the Retromax Kit. However, the packaging vectors are widely published and you may be able to find some information in the literature relevant to your model system (search scholar.google.com and keywords with Retromax and vector name, or keys words with packaging vector name and expression vector name ).

We would like to make sure that your pCL-Ampho Retrovirus Packaging Vector is compatible with the MSCV (Murine Stem Cell Virus) Retroviral Expression System.

The pCL-Ampho Retrovirus Packaging Vector NBP2-29541 should be compatible with a MSCV expression system. Please see the following publication that utilized MSCV2.2 with this packaging vector:

I was curious if you had a map for the pcl-ampho vector?

Unfortunately, we do not have a pCL-Ampho Vector Map in our records. This product was originally developed by Imgenex, a company we acquired at the beginning of last year, and it does not appear that the vector maps were supplied to us. We do have several publications referencing this product and you may find some of those publications useful: click on Reviews & Publications tab at NBP2-29541.

NBP2-29541 what is the sequence?

As stated under "Notes," the sequence of NBP2-29541 is not available. You can find out more information about this plasmid in the manual, including a plasmid map on page 22. The map is for pCl-ECO but the only difference between the Eco and Ampho plasmids is the envelope gene.

pCL-Ampho Retrovirus Packaging Vector

Name: pCL-Ampho Retrovirus Packaging Vector
Brand: Novus Biologicals
SKU: NBP2-29541

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29541

Submit a review

Citations (65)

Product Datasheet / COA / Protocols & Manuals

100% Guarantee

Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Hamster (Negative), Mammal

Applications

Immunocytochemistry/ Immunofluorescence, N/A

Product Specifications

Specificity

Ecotropic (MoMuLV) for mouse and rat cells only (not human)
Amphotropic (from 4070A MuLV) for most mammalian cells (but not hamster)
10A1 (MuLV) for most mammalian cells (including hamster)

Applications

Immunocytochemistry/ Immunofluorescence (reported in scientific literature (PMID 34082772))
Retroviral Production (reported in scientific literature (PMID 24311986))

Application Notes

For amplification, transform a suitable E. coli host such as HB101 or DH-5alpha and plate on LB plates containing 50-ug/ml ampicillin. For plasmid DNA preparation start culture from a single colony in LB medium containing 100 ug/ml ampicillin.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24311986).

Formulation, Preparation, and Storage

Formulation

10 ug in 20 ul sterile water.

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: pCL-Ampho Retrovirus Packaging Vector

The pCL-Ampho packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a cell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of 1 infectious particle/cell/day. Retrovirus tropism is determined at three levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. gp70 comes in three different flavors for gene therapy. The plasmid DNA can be amplified by transforming bacteria and growing them on LB/ampicillin (100 ug/ml) media.

Alternate Names

pCLAmpho, pCL-Ampho, pcl-ampho packaging vector, pCL-Ampho Retrovirus

Additional pCL-Ampho Retrovirus Packaging Vector Products

Product Documents for pCL-Ampho Retrovirus Packaging Vector

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download NBP2-29541 - Background and contents

Download NBP2-29541 - Protocol

Download Retroviral Expression System Manual

Download Protocol

COA

Product Specific Notices for pCL-Ampho Retrovirus Packaging Vector

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Citations
Reviews

Protocols
FAQs

pCL-Ampho Retrovirus Packaging Vector

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29541

Key Product Details

Species

Applications

Product Specifications

Specificity

Applications

Application Notes

Reactivity Notes

Formulation, Preparation, and Storage

Formulation

Concentration

Shipping

Storage

Background: pCL-Ampho Retrovirus Packaging Vector

Alternate Names

Additional pCL-Ampho Retrovirus Packaging Vector Products

Product Documents for pCL-Ampho Retrovirus Packaging Vector

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

Product Specific Notices for pCL-Ampho Retrovirus Packaging Vector

CONTACT INFORMATION

Stay Connected