Therapeutic Protein Charge Variant Characterization with Intact Mass and Peptide Mapping Following Microgram Preparative Capillary Isoelectric Focusing Electrophoresis Fractionation

The poster highlights the new MauriceFlex™ system, which offers high-resolution icIEF-based fractionation of protein charge variants for offline mass spectrometry (MS) characterization. This method allows for comprehensive MS analysis, including intact mass, subunit mass, and peptide mapping. The study demonstrates the fractionation of glenzocimab, with subsequent MS characterization for identifying modifications in its charge variants.