Ella™ Assays for Neuroscience Biomarker Research

First, see how Simple Plex assays on the EllaTM platform are used for Neuroscience research. Next, meet Ella and request pricing. Finally, choose the Simple Plex Assay that best meets your needs.

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Recently Launched Neuro Targets

Sensitive Data For Neuroscience Biomarker Research

Simple Plex™ assays on Ella™ give you the combination of sensitivity and reproducibility you need to advance your neuroscience biomarker research.

Simple Plex assays provide high-quality data on low abundance biomarkers in serum, plasma, and cerebrospinal fluid. Ella provides a fully integrated and automated workflow to give you data in just 90 minutes.

With Ella's automation, you also get an extremely high level of reproducibility and reliability. Simple Plex assays reduce manual operation and provide a rock-solid platform for your research.

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Dr. Yoav Noam discusses NF-L Assay Development

Powered By R&D Systems, A Bio-Techne Brand

With immunoassays developed by industry leader R&D Systems, Ella gives you the data you need to power your neuroscience research with a high level of automation, validation, and reliability.

 

Neurofilament Light (NF-L) in Cerebrospinal Fluid, Serum, and Plasma

Neurofilament light chain has been implicated as an important biomarker for a number of neurological disorders. The Simple Plex neurofilament light assay can quantify this biomarker in serum and plasma samples to provide highly robust and reproducible data.

The Simple Plex Assay Correlates with the Uman Diagnostics ELISA

 

Cerebrospinal fluid (CSF) NF-L levels measured using the Simple Plex Assay correlate with those quantified by the Uman Diagnostics ELISA. Samples were prepared according to the recommendations of the respective kit manufacturer. Levels of NF-L were measured within the linear range of each assay. The dilution factors were used to back-calculate the original concentration in the samples. These values were then plotted against each other, and a linear curve-fitting algorithm was applied.

The Simple Plex NF-L Assay is highly correlated with the Uman ELISA
The Simple Plex NF-L Assay is highly correlated with the Simoa ELISA

The Simple Plex Assay Correlates with the Simoa Bead-Based Assay

 

Plasma/serum NF-L levels measured using the Simple Plex Assay correlate highly with the Simoa assay. Samples were prepared according to the manufacturer's instructions. NF-L levels were measured within the linear range of each assay. The dilution factors were used to back-calculate the initial concentration in the samples. These values were then plotted against each other, and a linear curve-fitting algorithm was applied.

The Simple Plex NF-L assay has a mean CV of 9.7%

The Simple Plex NF-L Assay Has Excellent Inter-Assay Precision

 

The same plasma and serum samples, obtained from healthy donors, were prepared according to the Simple Plex NF-L Assay instructions and measured at three different sites. The mean sample coefficient of variation (CV) was less than 10%.

NF-L is Readily Detectable in CSF, Plasma and Serum

 

The ability to detect neurofilament light in the blood is important to allow minimally-invasive diagnosis and monitoring of disease progression. NF-L is readily detectable in cerebrospinal fluid (CSF), serum, EDTA plasma, and Heparin plasma. Serum and CSF samples are 100% detectable. Plasma samples are 90% detectable.

 

 

 

The Simple Plex assay detects NF-L in Serum, EDTA Plasma, Heparin Plasma and CSF
Simple plex neurofilament light chain assay data showing NF-L plasma levels in controls and ALS patients

The Simple Plex Assay Detects Elevated NF-L in Serum From ALS Patients

 

ALS patient samples were used as a model system to quantify NF-L levels since previous reports have established elevated NF-L levels in ALS serum. Healthy samples were analyzed on a 72x1 Simple Plex NF-L Cartridge and increased NF-L levels were observed in the ALS samples. Bars represent the median NF-L level ± the interquartile range. The statistical difference was evaluated using the nonparametric Mann-Whitney test with 19 to 20 samples per group.

 

 

Purchase the Simple Plex Human NF-L Cartridge

Peer-Reviewed Publications That Feature the Simple Plex NF-L Assay

  1. Giovannini et al. (2023) Neuro-glial degeneration in Status Epilepticus: Exploring the role of serum levels of Neurofilament light chains and S100B as prognostic biomarkers for short-term functional outcome Epilepsy & Behavior 140:109131. PMID: 36780777.

  2. Andreasson et al. (2023) Assessing the commutability of candidate reference materials for the harmonization of neurofilament light measurements in blood Clin Chem Lab Med 1. PMID: 36709509.

  3. Zingaropoli et al. (2022) Neuro-Axonal Damage and Alteration of Blood–Brain Barrier Integrity in COVID-19 Patients Cells 11:2480. PMID: 36010557.

  4. Kahouadji et al. (2022) Evaluation of serum neurofilament light in the early management of mTBI patients Clin Chem Lab Med 60:1234. PMID: 35511901.

  5. Halbgebauer et al. (2022) Comparison of CSF and serum neurofilament light and heavy chain as differential diagnostic biomarkers for ALS J Neurol Neurosurg Psychiatry 93:68. PMID: 34417339.

  6. Traub et al. (2022) Serum phosphorylated tau protein 181 and neurofilament light chain in cognitively impaired heart failure patients Alzheimer's Research & Therapy 14:149. PMID: 36217177.

  7. Ziemssen et al. (2022) Serum neurofilament light chain and initial severity of neurological disease predict the early neurological deterioration in Wilson’s disease Acta Neurologica Belgica 37:1074. PMID: 35114010.

  8. Notzel et al. (2022) Ella versus Simoa Serum Neurofilament Assessment to Monitor Treatment Response in Highly Active Multiple Sclerosis Patients Int J Mol Sci 23:12361. PMID: 36293227.

  9. Massa et al. (2022) Exploring the brain metabolic correlates of process-specific CSF biomarkers in patients with MCI due to Alzheimer's disease: preliminary data Neurobiology of Aging 117:212-221. PMID: 35780561.

  10. Zimmerman et al. (2022) White matter alterations in chronic MDMA use: Evidence from diffusion tensor imaging and neurofilament light chain blood levels NeuroImage 36:103191. PMID: 36126513.

  11. GIovannini et al. (2022) Serum neurofilament light as biomarker of seizure-related neuronal injury in status epilepticus Epilepsia 63:e23. PMID: 34806176.

  12. Guasp et al. (2022) CSF Biomarkers in COVID-19 Associated Encephalopathy and Encephalitis Predict Long-Term Outcome Front. Immunol PMID: 35479062.

  13. Freigang et al. (2022) Glial fibrillary acidic protein in cerebrospinal fluid of patients with spinal muscular atrophy Ann Clin Transl Neurol PMID: 35951535.

  14. Gauthier et al. (2021) Comparison of Simoa TM and Ella TM to assess serum neurofilament-light chain in multiple sclerosis Ann Clin Transl Neurol 5:1150. PMID: 33830650.

  15. Rojas et al. (2021) Plasma Neurofilament Light for Prediction of Disease Progression in Familial Frontotemporal Lobar Degeneration Neurology PMID: 33827960.

  16. Bittner et al. (2021) The potential of serum neurofilament as biomarker for multiple sclerosis Brain 144:2954. PMID: 34180982.

Highly Sensitive and Reproducible NF-L Measurements

With the Simple Plex NF-L assay on Ella you get measurements on an automated, robust and reproducible immunoassay platform. In our application note we demonstrate how Ella provides highly reproducible NF-L measurements even at the low levels detected in serum and plasma. Learn how to bring your NF-L research to the next level with Ella.

Download the NF-L App Note

 

 

NF-L and NF-H as Important Indicators of Neurodegeneration and Preeclampsia

Neurofilament heavy and light chains are associated with a number of pathological conditions, and both individually and in conjunction are promising better diagnosis and monitoring of a number of diseases. Learn how researchers at Beth Israel Deaconess Medical Center, Harvard Medical School, and Cedars-Sinai in collaboration with Bio-Techne are exploring the promise of NF-L and NF-H as biomarkers. Download our poster on NF-L and NF-H as emerging biomarkers.

Download the NF-L/NF-H Poster

 

 

Biomarkers Validated for Cerebrospinal Fluid

Need better biomarker data from your cerebrospinal fluid (CSF) samples? We've got neuroscience biomarker assays that have been fully validated for measurement in CSF. Have precious samples and need to measure multiple biomarkers? Talk to our specialists about creating a custom panel for your research.

Neuroscience Biomarkers Validated for CSF
BAFF Fractalkine IL-15 TACI
CCL2/MCP-1 GFAP LIGHT TIMP-1
CD21 ICAM-1 Lipo-2 TNFR1
CD27 IFNg 3rd Gen NF-H TREM-2
CHI3L1/YKL-40 IL-6 NF-L VCAM-1
CXCL10 IL-6 2nd Gen Osteopontin 2nd Gen  
Ferritin IL-8 Procalcitonin

 

 

Simple Plex Neuroscience Biomarker Research in Literature

Learn how researchers are using neurofilament and neuroinflammation biomarker assays on Ella in their research.

  1. Gauthier et al. (2021) Comparison of Simoa TM and Ella TM to assess serum neurofilament-light chain in multiple sclerosis Ann.Clin.Transl.Neurol. 8:1141. PMID: 33830650.

  2. Minikel et al. (2020) Prion protein lowering is a disease-modifying therapy across prion disease stages, strains and endpoints Nucleic Acids Res. 48:10615. PMID: 32776089.

  3. O Scott et al. (2020) The Inflammasome Adaptor Protein ASC in Mild Cognitive Impairment and Alzheimer's Disease Int.J.Mol.Sci . 21:4674. PMID: 32630059.

  4. Darrad et al. (2019) Neurofilament as a potential biomarker for spinal muscular atrophy Ann.Clin.Transl.Neurol . 6:932. PMID: 31139691.

  5. Syed et al. (2018) Defective Inflammatory Pathways in Never-Treated Depressed Patients Are Associated with Poor Treatment Response Neuron 99:914. PMID: 30146307.

  6. Keane et al. (2018) Inflammasome Proteins As Biomarkers of Multiple Sclerosis Front. Neurol 9:135. PMID: 29615953.

  7. Yeung et al. (2016) Evaluation of highly sensitive immunoassay technologies for quantitative measurements of sub-pg/mL levels of cytokines in human serum J.Immunol. Methods 437:53. PMID: 27555238.

Simple Plex Applications

sars cov-2 virus

COVID-19 Research

Quantify cytokine and pro-inflammatory responses to SARS-CoV-2 infection in 90 minutes using an automated, multianalyte format.

joint inflammation

Inflammation Research

Simple Plex assays enable rapid and reproducible quantification of inflammatory responses such as cytokine release syndrome and sepsis. 

immune cells attack cancer cell

Cancer Biomarker Research

Simple Plex assays for cancer research have the sensitivity to profile low abundance cancer biomarkers.

neuronal network

Neuroscience Research

Measure key neuroscience biomarkers such as neurofilament light (NF-L) and neurofilament heavy (NF-H) in CSF, plasma, and serum.

Cell and gene therapy

Cell Therapy Research and Development

The Simple Plex HEK 293 HCP 3G assay on the Ella platform offers broad detection coverage of this host cell protein (HCP).

protein bioprocessing workflow

Bioprocess Testing

Iterate your workflow faster with Simple Plex CHO HCP 3G-1 assays for process-related impurity testing.

preclinical scientist pipetting in the lab

Preclinical Research

With a four-log range of detection, Simple Plex assays have the sensitivity to pick up even very low picogram-per-milliliter levels of endogenous mouse or rat analytes.

AAV2 virus for simple plex

Viral Titer Assays

Get highly reproducible adeno-associated virus and lentivirus titer data with no manual steps in just 90 minutes.

Simple Plex Cartridge Denominations

Automated ELISA platform assay options including singleplex, multianalyte and multiplex assay cartridges

 

 

  16x1 32x1 16x2 16x3 16x4 32x2 72x1 32x3 32x4 32x5 32x6 32x7 32x8
Samples 16 32 16 16 16 32 72 32 32 32 32 32 32
Plex 1 1 2 3 4 2 1 3 4 5 6 7 8
Replicates 3 3 3 3 3 3 3 3 3 2 2 2 2
Reportable Results 16 32 32 48 64 64 72 96 128 160 192 224 256
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