Annexin V Apoptosis Kit [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92655

Novus Biologicals, part of Bio-Techne
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NBP1-92655-100Tests
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Key Product Details

Species

Human

Applications

Flow Cytometry

Kit Type

Apoptosis Kit

Conjugate

Biotin

Product Summary for Annexin V Apoptosis Kit [Biotin]

Annexin V exhibits antiphospholipase activity and binds to phosphatidylserine. Biotin labelling allows simple direct detection by FACS analysis. The Annexin V-Biotin Kit can be used to detect phosphatidylserine on the outer leaflet of the cell membrane using flow cytometry. Counterstaining by propidium iodide allows the discrimination of apoptotic cells.

Kit Contents for Annexin V Apoptosis Kit [Biotin]

  • Annexin V-Biotin protein
  • Binding Buffer (4x)
  • Propidium Iodide (20 ug/mL)

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Storage of components varies. See protocol for specific instructions.

Background: Annexin V

Annexins are a family of calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS) to identify apoptotic cells. In healthy cells, PS is predominantly located along the cytosolic side of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution in the phospholipid bilayer and translocates to the extracellular membrane, which is detectable with fluorescently labeled Annexin V. In early stages of apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI) and 7-AAD, therefore cells which display only Annexin V staining (PI/7-AAD negative) are in early stages of apoptosis. During late-stage apoptosis, loss of cell membrane integrity allows Annexin V binding to cytosolic PS, as well as cell uptake of PI and 7-AAD. Annexin V staining, paired with 7-AAD or PI is widely used to identify apoptotic stages by flow cytometry.

Alternate Names

ANX5, ANXA5, ANXV

Gene Symbol

ANXA5

Additional Annexin V Products

Product Documents for Annexin V Apoptosis Kit [Biotin]

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Annexin V Apoptosis Kit [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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