beta Galactosidase Activity Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24551

Novus Biologicals, part of Bio-Techne
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NBP3-24551
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell samples, animal tissue and plant tissue

Sensitivity

16.19 U/L

Assay Range

16.19 - 1200 U/L

View all beta Galactosidase Activity Assays »

Product Specifications

Kit Type

Activity Assay Kit (Colorimetric)

Reactivity

Non-species specific

Description

Detection instrument: Microplate reader (390 - 410 nm, optimum wavelength: 400 nm)
Assay time: 70 min

Precision

Intra-Assay Precision (Precision within an assay) %CV < 3

Inter-Assay Precision (Precision between assays) %CV < 6

Recovery for beta Galactosidase Activity Assay Kit (Colorimetric)

Recovery

Average recovery rate: 101%

Scientific Data Images for beta Galactosidase Activity Assay Kit (Colorimetric)

beta Galactosidase Activity Assay Kit (Colorimetric)

beta Galactosidase Activity Assay Kit (Colorimetric) [NBP3-24551] -

beta Galactosidase Activity Assay Kit (Colorimetric) [NBP3-24551] - Standard Reference Curve
beta Galactosidase Activity Assay Kit (Colorimetric)

beta Galactosidase Activity Assay Kit (Colorimetric) [NBP3-24551] -

beta Galactosidase Activity Assay Kit (Colorimetric) [NBP3-24551] - Assay Example

Kit Contents for beta Galactosidase Activity Assay Kit (Colorimetric)

  • Microplate
  • Plate Sealer
  • Reagent 1: Buffer Solution
  • Reagent 2: Substrate
  • Reagent 3: Activator Agent
  • Reagent 4: Chromogenic Agent
  • Reagent 5: 20 mmol/L Standard Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: beta Galactosidase

Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.

Alternate Names

EC 3.2.1.23; b-gal

Additional beta Galactosidase Products

Product Documents for beta Galactosidase Activity Assay Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for beta Galactosidase Activity Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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