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Caspase-8 Activity Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54817

Novus Biologicals, part of Bio-Techne
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NBP2-54817-25Assays

Key Product Details

Sample Type & Volume Required Per Well

Cell and tissue lysates

Product Specifications

Kit Type

Activity Assay Kit (Colorimetric)

Reactivity

Human, Mouse, Rat, Mammal

Description

Simple and convenient colorimetric assay to measure Caspase-8/FLICE activity within 1-2 hrs.
    Detection method: Absorbance (400 or 405 nm)Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.


This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab39700)

Scientific Data Images for Caspase-8 Activity Assay Kit (Colorimetric)

Caspase-8 Activity Assay Kit (Colorimetric) [NBP2-54817] - The assay was performed on murine insulinoma cell line following Novu's protocol.

Kit Contents for Caspase-8 Activity Assay Kit (Colorimetric)

  • 2X Reaction Buffer
  • Cell Lysis Buffer
  • Dilution Buffer
  • DTT (1 M)
  • IETD-pNA (4 mM)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Caspase-8

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The FLICE/Caspase-8 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate IETD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405 nm.

Alternate Names

CASP8, Caspase8, Mch5

Gene Symbol

CASP8

Additional Caspase-8 Products

Product Documents for Caspase-8 Activity Assay Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Caspase-8 Activity Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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