Catalase Activity Assay Kit (Fluorometric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24478

Novus Biologicals, part of Bio-Techne
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NBP3-24478
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and animal tissue

Sensitivity

0.01 U/L

Assay Range

0.01 - 6.51 U/L

View all Catalase Activity Assays »

Product Specifications

Kit Type

Activity Assay Kit (Fluorometric)

Reactivity

Non-species specific

Description

Detection instrument: Fluorescence microplate reader (Ex/Em = 535 nm/587 nm)
Assay time: 30 min

Precision

Intra-Assay Precision (Precision within an assay) %CV < 3.4

Inter-Assay Precision (Precision between assays) %CV < 6.8

Recovery for Catalase Activity Assay Kit (Fluorometric)

Recovery

Average recovery rate: 92%

Scientific Data Images for Catalase Activity Assay Kit (Fluorometric)

Catalase Activity Assay Kit (Fluorometric)

Catalase Activity Assay Kit (Fluorometric) [NBP3-24478] -

Catalase Activity Assay Kit (Fluorometric) [NBP3-24478] - Standard Reference Curve
Catalase Activity Assay Kit (Fluorometric)

Catalase Activity Assay Kit (Fluorometric) [NBP3-24478] -

Catalase Activity Assay Kit (Fluorometric) [NBP3-24478] - Assay Example

Kit Contents for Catalase Activity Assay Kit (Fluorometric)

  • Black Microplate
  • Plate Sealer
  • Reagent 1: Buffer Solution
  • Reagent 2: Substrate
  • Reagent 3: Probe Solution
  • Reagent 4: Enzyme Reagent
  • Reagent 5: 1 mol/L H2O2 Standard Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Catalase

Human catalase is an heme-containing peroxisomal enzyme that breaks down hydrogen peroxide to water and oxygen; it is implicated in ethanol metabolism, inflammation, apoptosis, aging and cancer. The 1.5A resolution human enzyme structure, both with and without bound NADPH, establishes the conserved features of mammalian catalase fold and assembly, implicates Tyr370 as the tyrosine radical, suggests the structural basis for redox-sensitive binding of cognate mRNA via the catalase NADPH binding site, and identifies an unexpectedly substantial number of water-mediated domain contacts (1). Because catalase has a low affinity for H2O2, it has been suggested that glutathione peroxidase clears most H2O2 within the erthrocyte and that catalase is of little import. It has been hypothesized that erythrocyte catalase might function to protect heterologous somatic cells against challenge by high levels of exogenous H2O2 (e.g., in areas of inflamation) (2).

Alternate Names

Cas1, CAT, Cs-1

Gene Symbol

CAT

Additional Catalase Products

Product Documents for Catalase Activity Assay Kit (Fluorometric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Catalase Activity Assay Kit (Fluorometric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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