Glutamine Synthetase Activity Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24495

Novus Biologicals, part of Bio-Techne
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NBP3-24495
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plant tissue and animal tissue

Sensitivity

8.84 U/L

Assay Range

8.84 - 321.05 U/L

View all Glutamine Synthetase Activity Assays »

Product Specifications

Kit Type

Activity Assay Kit (Colorimetric)

Reactivity

Non-species specific

Description

Detection instrument: Microplate reader (330 - 350 nm, optimum wavelength: 340 nm)
Assay time: 15 min

Precision

Intra-Assay Precision (Precision within an assay) %CV < 2.5

Inter-Assay Precision (Precision between assays) %CV < 5.8

Recovery for Glutamine Synthetase Activity Assay Kit (Colorimetric)

Recovery

Average recovery rate: 104%

Scientific Data Images for Glutamine Synthetase Activity Assay Kit (Colorimetric)

Glutamine Synthetase Activity Assay Kit (Colorimetric)

Glutamine Synthetase Activity Assay Kit (Colorimetric) [NBP3-24495] -

Glutamine Synthetase Activity Assay Kit (Colorimetric) [NBP3-24495] - Assay Example

Kit Contents for Glutamine Synthetase Activity Assay Kit (Colorimetric)

  • Plate Sealer
  • Reagent 1: Extracting Solution
  • Reagent 2: Buffer Solution
  • Reagent 3: Substrate A
  • Reagent 4: Substrate B
  • Reagent 5: Chromogenic Agent
  • Reagent 6: Standard
  • UV Microplate

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Glutamine Synthetase

Glutamine synthetase is part of the glutamine synthetase family. Specifically, Glutamine synthetase catalyzes the condensation of glutamate and ammonia to form glutamine, during the metabolism of nitrogen. Ammonia incorporation in animals occurs through the actions of glutamate dehydrogenase and glutamine synthase. Glutamate plays the central role in mammalian nitrogen flow, serving as both a nitrogen donor and nitrogen acceptor.

Glutamine synthetase antibodies are useful tools for studying nitrogen metabolism and regulation.

Alternate Names

cell proliferation-inducing protein 59, EC 4.1.1.15, EC 6.3.1.2, GLNSPIG59, Glutamate decarboxylase, glutamate-ammonia ligase, Glutamate--ammonia ligase, glutamate-ammonia ligase (glutamine synthase), glutamine synthase, glutamine synthetase, GS, PIG43, proliferation-inducing protein 43

Gene Symbol

GLUL

Additional Glutamine Synthetase Products

Product Documents for Glutamine Synthetase Activity Assay Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Glutamine Synthetase Activity Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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