Glutathione Reductase Activity Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24498

Novus Biologicals, part of Bio-Techne
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NBP3-24498
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue and cell samples

Sensitivity

6.2 U/L

Assay Range

6.2 - 320 U/L

View all Glutathione Reductase Activity Assays »

Product Specifications

Kit Type

Activity Assay Kit (Colorimetric)

Reactivity

Non-species specific

Description

Detection instrument: Spectrophotometer (340 nm)
Assay time: 90 min

Precision

Intra-Assay Precision (Precision within an assay) %CV < 2.1

Inter-Assay Precision (Precision between assays) %CV < 2.5

Recovery for Glutathione Reductase Activity Assay Kit (Colorimetric)

Recovery

Average recovery rate: 100%

Scientific Data Images for Glutathione Reductase Activity Assay Kit (Colorimetric)

Glutathione Reductase Activity Assay Kit (Colorimetric)

Glutathione Reductase Activity Assay Kit (Colorimetric) [NBP3-24498] -

Glutathione Reductase Activity Assay Kit (Colorimetric) [NBP3-24498] - Standard Reference Curve
Glutathione Reductase Activity Assay Kit (Colorimetric)

Glutathione Reductase Activity Assay Kit (Colorimetric) [NBP3-24498] -

Glutathione Reductase Activity Assay Kit (Colorimetric) [NBP3-24498] - Assay Example

Kit Contents for Glutathione Reductase Activity Assay Kit (Colorimetric)

  • Reagent 1: Buffer Solution
  • Reagent 2: Substrate
  • Reagent 3: Enzyme Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Glutathione Reductase

Glutathione reductase (GR) is a member of pyridine nucleotide-disulfideoxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione(GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+ ->2GSH + NADP+. In mosteukaryotic cells, GR maintains the ratio of [GSH]/[GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis.

Alternate Names

GLUR, GRase, GRD1, GSR, HEL-752

Gene Symbol

GSR

Additional Glutathione Reductase Products

Product Documents for Glutathione Reductase Activity Assay Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Glutathione Reductase Activity Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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