Myeloperoxidase/MPO Activity Assay Kit (Fluorometric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24524

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and animal tissue

Sensitivity

0.001 U/L

Assay Range

0.001 - 1.26 U/L

View all Myeloperoxidase/MPO Activity Assays »

Product Specifications

Kit Type

Activity Assay Kit (Fluorometric)

Reactivity

Non-species specific

Description

Detection instrument: Fluorescence microplate reader (Ex/Em = 535 nm/587 nm)
Assay time: 20 min

Precision

Intra-Assay Precision (Precision within an assay) %CV < 1

Inter-Assay Precision (Precision between assays) %CV < 5.4

Scientific Data Images for Myeloperoxidase/MPO Activity Assay Kit (Fluorometric)

Myeloperoxidase/MPO Activity Assay Kit (Fluorometric)

Myeloperoxidase/MPO Activity Assay Kit (Fluorometric) [NBP3-24524] -

Myeloperoxidase/MPO Activity Assay Kit (Fluorometric) [NBP3-24524] - Standard Reference Curve
Myeloperoxidase/MPO Activity Assay Kit (Fluorometric)

Myeloperoxidase/MPO Activity Assay Kit (Fluorometric) [NBP3-24524] -

Myeloperoxidase/MPO Activity Assay Kit (Fluorometric) [NBP3-24524] - Assay Example

Kit Contents for Myeloperoxidase/MPO Activity Assay Kit (Fluorometric)

  • Black Microplate
  • Plate Sealer
  • Reagent 1: Buffer Solution
  • Reagent 2: Probe
  • Reagent 3: Substrate
  • Reagent 4: Inhibitor
  • Reagent 5: 25 umol/L Resorufin Standard

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Myeloperoxidase/MPO

FUNCTION: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. MPO is an important marker for myeloid cells, from the promyelocyte stage and to the mature forms. CATALYTIC ACTIVITY: Donor + H2O2 = oxidized donor + 2 H2O. CATALYTIC ACTIVITY: Cl- + H2O2 = HOCl + 2 H2O. COFACTOR: Binds 1 calcium ion per heterodimer. COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer. SUBUNIT: Tetramer of two light chains and two heavy chains. SUBCELLULAR LOCATION: Lysosome. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. DISEASE: Defects in MPO are the cause of myeloperoxidase deficiency (MPD). MPD is an autosomal recessive defect that results in disseminated candidiasis. SIMILARITY: Belongs to the peroxidase family. XPO subfamily.

Alternate Names

MPO

Gene Symbol

MPO

Additional Myeloperoxidase/MPO Products

Product Documents for Myeloperoxidase/MPO Activity Assay Kit (Fluorometric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Myeloperoxidase/MPO Activity Assay Kit (Fluorometric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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