TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24536

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

serum, plasma, urine, animal tissue and cell samples

Sensitivity

9.22 U/L

Assay Range

9.22 - 100 U/L

View all TRACP/PAP/ACP5 Activity Assays »

Product Specifications

Kit Type

Activity Assay Kit (Colorimetric)

Reactivity

Non-species specific

Description

Detection instrument: Microplate reader (410 - 415 nm, optimum wavelength: 405 nm)
Assay time: 25 min

Precision

Intra-Assay Precision (Precision within an assay) %CV < 2

Inter-Assay Precision (Precision between assays) %CV < 2.3

Recovery for TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric)

Recovery

Average recovery rate: 101%

Scientific Data Images for TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric)

TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric)

TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric) [NBP3-24536] -

TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric) [NBP3-24536] - Standard Reference Curve
TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric)

TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric) [NBP3-24536] -

TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric) [NBP3-24536] - Assay Example

Kit Contents for TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric)

  • Microplate
  • Plate Sealer
  • Reagent 1: Buffer Solution
  • Reagent 2: Substrate
  • Reagent 3: Tartaric Acid Solution
  • Reagent 4: Standard Substance
  • Reagent 5: Chromogenic Agent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: TRACP/PAP/ACP5

TRACP is an iron-containing, 35 kDa glycoprotein that hydrolyses phosphomonoesters. It is produced as a monomer by osteoclasts and is cleaved by cathepsins to create a disulfide-linked dimer that acts on bone matrix phosphoserine-containing proteins in lysosomal vesicles. Following phosphate cleavage, TRACP is released from the cell where it serves as a marker of bone resorption.

Long Name

Tartrate Resistant Acid Phosphatase 5

Alternate Names

ACP5, TRACP-5b, TTRRAP

Gene Symbol

ACP5

Additional TRACP/PAP/ACP5 Products

Product Documents for TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRACP/PAP/ACP5 Activity Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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