What wavelength should be used to read the chemiluminescent plate?

The peroxyglow substrates used in the chemiluminescent kit are in the visible light range, so the plate reader should not filter the light source or the detector. The detector measures light intensity without specificity for a particular wavelength. The results are in all the wavelengths and report the amount of detected emitted light in relative light units or RLU. We recommend using a luminometer that is used for luciferase assays.

What is the maximum DMSO concentration in samples that can interfere with the assay?

Maximum DMSO concentration for inhibitor vehicles has not been evaluated. It is recommended to include a DMSO only control in the assay.

Does this kit contain the full-length or truncated form of PARP?

This is the full length PARP1 containing a short ubiquitin tag on the amino terminus.

Universal Chemiluminescent PARP Assay - Histone Coated Wells

Name: Universal Chemiluminescent PARP Assay - Histone Coated Wells
Brand: R&D Systems
SKU: 4676-096-K

R&D Systems, part of Bio-Techne | Catalog # 4676-096-K

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Product Datasheet / COA

For the in vitro screening of candidate PARP-1 inhibitors

Key Product Details

Features:	Ideal for the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.
Key Benefits:	Chemiluminescent readout 96 strip-well format Sensitive – detects 10 mU PARP /well Assay Time ~3 hrs

Technical Data
Citations / Reviews / FAQs

Summary for Universal Chemiluminescent PARP Assay - Histone Coated Wells

Why Use the Universal Chemiluminescent PARP Assay Kit?

This ELISA based assay detects biotinylated poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. The addition of Strep-HRP (biotin-binding protein) and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with PARP-1 activity.

Product Specifications

For the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.

Kit Contents for Universal Chemiluminescent PARP Assay - Histone Coated Wells

3-Aminobenzamide
Human PARP 1 Enzyme, HSA
20X PARP Buffer
10X PARP Cocktail
10X Activated DNA
10X Strep-Diluent
PeroxyGlow™ A
PeroxyGlow™ B
Histone-Coated White Strip Well Plate
Strep-HRP

Product Specifications for Universal Chemiluminescent PARP Assay - Histone Coated Wells

Species

Multi-species

Preparation & Storage

Shipping Conditions	The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage	Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

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