VasoTACS In Situ Apoptosis Detection Kit

Name: VasoTACS In Situ Apoptosis Detection Kit
Brand: R&D Systems
SKU: 4826-30-K

R&D Systems, part of Bio-Techne | Catalog # 4826-30-K

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Citations (4)

Product Datasheet / COA

In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded vascular cells and tissues.

Key Product Details

Features:	Provides an effective in situ method for identifying apoptosis in vascular samples using an optimized TUNEL based assay.
Key Benefits:	Performance tested on vascular tissues. Includes both Proteinase K and exclusive Cytonin non-enzymatic permeabilization reagents. Helps resolve unique problems encountered when detecting apoptotic vascular cells.

Technical Data
Citations / Reviews

Summary for VasoTACS In Situ Apoptosis Detection Kit

Why Use the VasoTACS In Situ Apoptosis Detection Kit?

This kit allows the user to successfully label apoptotic cells, including endothelial and smooth muscle cells, throughout the vascular system. Similar to the CardioTACS Kit, this kit is also based on DNA end-labeling using terminal deoxynucleotidyl transferase (TdT) and a modified nucleotide that is subsequently detected using our TACS Blue Label detection system with Red Counterstain C. Specifically, VasoTACS™ has been tested and optimized to help resolve the unique problems encountered when detecting apoptotic vascular cells.

Kit Contents for VasoTACS In Situ Apoptosis Detection Kit

Proteinase K
Streptavidin-HRP
TACS-Blue Label
Blue Strep-HRP Diluent
TACS-Nuclease
TACS-Nuclease Buffer
Red Counterstain C
TACS 2 TdT Labeling Buffer
TACS 2 TdT Stop Buffer
TACS 2 TdT dNTP
TdT Enzyme
50x Manganese Cation
Cytonin

Product Specifications for VasoTACS In Situ Apoptosis Detection Kit

Species

Multi-species

Scientific Data Examples for VasoTACS In Situ Apoptosis Detection Kit

	Visualization of Apoptotic Cells in Vascular Tissue using the VasoTACS In Situ Apoptosis Detection Kit. TIssue from the small artery of rat was formalin-fixed and paraffin-embedded following the initiation of drug-induced apoptosis. The tissue was processed according to the protocol in the VasoTACS Kit (Catalog # 4826-30-K) and visualized by light microscopy. Brown stained nuclei following counterstaining with Red Counterstain C indicated apoptotic cells. Data was provided courtesy of Dr. Jun Zhang, FDA.

Preparation & Storage

Shipping Conditions	The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

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Product Documents for VasoTACS In Situ Apoptosis Detection Kit

Product Datasheets

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