14-3-3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2140

Novus Biologicals, part of Bio-Techne
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NB100-2140
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all 14-3-3 Antibodies »

Product Specifications

Immunogen

Synthetic peptide mapping to the amino terminal domain of human 14-3-3.

Localization

Cytoplasmic

Specificity

Broadly reactive among the 14-3-3 family, will recognize alpha, beta, zeta (delta) and theta 14-3-3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 14-3-3 Antibody

Western Blot: 14-3-3 Antibody [NB100-2140]

Western Blot: 14-3-3 Antibody [NB100-2140]

Western Blot: 14-3-3 Antibody [NB100-2140] - Analysis of 14-3-3 expression in Hela (A) and Jurkat (B) whole cell lysates.

Applications for 14-3-3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:400

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, with 0.2% gelatin

Preservative

0.1% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: 14-3-3

14-3-3 family of proteins is composed of seven isotypes, beta, gamma, zeta, epsilon, tau, eta, and sigma, that play critical roles in cell signaling events that control cell cycle progression, transcriptional alterations, and apoptosis (1-4). 14-3-3 proteins were the first signaling molecules to be identified as specific phosphoserine/threonine binding proteins (1). 14-3-3 can serve as a direct regulator of its target by altering the function of the protein (3). 14-3-3 protein targets include the signalling intermediates Raf, MEKK, PI-3 kinase and IRS-1, cell cycle proteins CDK2, Wee1, and Cdc25, and apoptosis proteins BAD and ASK-1 (3,4).

Alternate Names

1433

Entrez Gene IDs

10971 (Human)

Gene Symbol

YWHAQ

UniProt

P27348

Additional 14-3-3 Products

Product Documents for 14-3-3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 14-3-3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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