14-3-3 beta Antibody (05)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06157
![Western Blot: 14-3-3 beta Antibody (05) [NBP3-06157]](https://resources.bio-techne.com/images/products/14-3-3-beta-Antibody-05-Western-Blot-NBP3-06157-img0001.jpg "Western Blot: 14-3-3 beta Antibody (05) [NBP3-06157]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Cynomolgus Monkey
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 05
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for 14-3-3 beta Antibody (05)
Immunogen
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Cynomolgus 14-3-3 beta (Uniprot#: Q4R572-2; Met 2-Asn 244).
Reactivity Notes
Has cross-reactivity in ELISA with:
Human YWHAB
No cross-reactivity in ELISA with:
E.coli cell lysate
Human YWHAB
No cross-reactivity in ELISA with:
E.coli cell lysate
Specificity
Has cross-reactivity in ELISA with: Human YWHABNo cross-reactivity in ELISA with: E.coli cell lysate
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for 14-3-3 beta Antibody (05)
Western Blot: 14-3-3 beta Antibody (05) [NBP3-06157]
Western Blot: 14-3-3 beta Antibody (05) [NBP3-06157] - Anti-YWHAB mouse monoclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Mouse IgG H&L (Dylight800) at 115000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:32 kDa
14-3-3 beta Antibody (05) [NBP3-06157] - cynoYWHAB was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate Lane B: 0.5 mg Hela Whole Cell Lysate 2 uL anti-cyno YWHAB mouse monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-cyno YWHAB mouse monoclonal antibody, at 1:200 dilution Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 32 kDa Observed band size: 32 kDa
Applications for 14-3-3 beta Antibody (05)
Application
Recommended Usage
ELISA
1:1000-1:2000
Immunoprecipitation
4-6 uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: 14-3-3 beta
Alternate Names
1433 beta, GW128, HS1, YWHAB
Gene Symbol
YWHAB
Additional 14-3-3 beta Products
Product Documents for 14-3-3 beta Antibody (05)
Product Specific Notices for 14-3-3 beta Antibody (05)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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