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14-3-3 beta Antibody (05) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06157CL1

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Cynomolgus Monkey

Applications

ELISA, Immunoprecipitation, Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG2B Clone # 05

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for 14-3-3 beta Antibody (05) [CoraFluor™ 1]

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Cynomolgus 14-3-3 beta (Uniprot#: Q4R572-2; Met 2-Asn 244).

Reactivity Notes

Has cross-reactivity in ELISA with:

Human YWHAB

No cross-reactivity in ELISA with:

E.coli cell lysate

Specificity

Has cross-reactivity in ELISA with: Human YWHABNo cross-reactivity in ELISA with: E.coli cell lysate

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for 14-3-3 beta Antibody (05) [CoraFluor™ 1]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: 14-3-3 beta

14-3-3 proteins constitute a highly conserved family of homo- and heterodimeric proteins present in high abundance in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs containing phosphorylated serine or threonine residues. There are at least seven distinct 14-3-3 genes in vertebrates: alpha/beta, epsilon, eta, gamma, theta, sigma, and zeta/delta.

Alternate Names

1433 beta, GW128, HS1, YWHAB

Gene Symbol

YWHAB

Additional 14-3-3 beta Products

Product Documents for 14-3-3 beta Antibody (05) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 14-3-3 beta Antibody (05) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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